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. 2023 Aug 29;11(9):2183. doi: 10.3390/microorganisms11092183

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Microscopic observation of sebocytes cultured after fixation and labeling with Nile Red and Hoechst. Panel (A): Sebocytes alone. Panel (B): Sebocytes with S. epidermidis. Panel (C): Sebocytes with C. acnes. Panel (D): Sebocytes with S. epidermidis and C. acnes. Pictures were taken via an epifluorescence microscope. Hoechst marks the nuclei in blue, while Nile Red reveals neutral lipids in green and total lipids in red. Superimposing the two colors highlights the lipid droplets in yellow. Scale bar: 100 µm.