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. 1987 Sep;85(1):87–90. doi: 10.1104/pp.85.1.87

Partial Purification of Gibberellin Oxidases from Spinach Leaves 1

Sarah J Gilmour 1,2, Anthony B Bleecker 1, Jan A D Zeevaart 1
PMCID: PMC1054208  PMID: 16665690

Abstract

Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA12 → GA53 → GA44 → GA19 → GA20. Two of these, GA53 oxidase and GA19 oxidase, were separable from the other two, GA44 oxidase and GA12 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as determined by gel filtration HPLC are: GA12 13-hydroxylase, 28,400; GA53 oxidase, 42,500; GA44 oxidase, 38,100; GA19 oxidase, 39,500. GA44 oxidase was purified approximately 100-fold in 0.3% yield by a combination of ammonium sulfate fractionation, anion exchange HPLC, phenyl-Sepharose chromatography and gel filtration HPLC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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