Figure 4. Isoprenoid biosynthesis suppressor mutations require UppS^UN to confers increased resistance to SDS/EDTA in 17978 ΔmlaF strains.

(A) Schematic of the isoprenoid biosynthetic pathway with key genes highlighted in red. (B and C) WT and mutant strains were serially diluted before spotting on LB plates without (B) and with (C) 0.01 % SDS and 0.15 mM EDTA. Data are representative of 4 experiments with a total of 8 biological replicates. (D to G) VU (D and E) and UN (F and G) WT and mutant strains were grown in LB without (D and F) or with (E and G) 0.01% SDS and 0.175 mM EDTA. n=3, data are means ± SEM.