Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1988 Jan;86(1):28–31. doi: 10.1104/pp.86.1.28

Changes in Free and Conjugated Indole 3-Acetic Acid and Abscisic Acid in Young Cotton Fruits and Their Abscission Zones in Relation to Fruit Retention during and after Moisture Stress

Gene Guinn 1,2, Donald L Brummett 1,2
PMCID: PMC1054422  PMID: 16665881

Abstract

Experiments were conducted with field-grown cotton (Gossypium hirsutum L.) in 1985 and 1986 to determine effects of water deficit on levels of conjugated indole 3-acetic acid (IAA) and abscisic acid (ABA) in young fruits (bolls) and their abscission zones in relation to boll retention. Tissues were harvested three times during an irrigation cycle in 1985. They were harvested twice during an irrigation cycle and once after irrigation in 1986 to determine extent of recoveries of measured parameters. As reported earlier, the free IAA content of abscission zones decreased with moisture stress. Irrigation caused a partial recovery in free IAA content of abscission zones and caused a partial recovery in rate of boll retention. In contrast to free IAA, conjugated IAA increased with water deficit, both in 3-day-old bolls and in their abscission zones. Bolls contained much more ester IAA than their abscission zones. Some, but not all, of the increase in ester IAA in bolls during moisture stress could have come from a conversion of amide-linked IAA. Amide IAA decreased slightly during stress and increased after irrigation, but the concentration was low relative to ester IAA. Free and conjugated ABA both increased during stress and decreased after irrigation. However, the concentration of conjugated ABA remained relatively high in abscission zones. Ester IAA, being more resistant than free IAA to enzymic destruction during stress, may hasten recovery of fruit retention after relief of stress by providing a source of free IAA in abscission zones to inhibit continued abscission.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeles F. B. Abscission: role of cellulase. Plant Physiol. 1969 Mar;44(3):447–452. doi: 10.1104/pp.44.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bandurski R. S., Schulze A. Concentration of Indole-3-acetic Acid and Its Derivatives in Plants. Plant Physiol. 1977 Aug;60(2):211–213. doi: 10.1104/pp.60.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beyer E. M., Morgan P. W. Effect of ethylene on the uptake, distribution, and metabolism of indoleacetic Acid-1-C and -2-C and naphthaleneacetic Acid-1-C. Plant Physiol. 1970 Jul;46(1):157–162. doi: 10.1104/pp.46.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boyer G. L., Zeevaart J. A. Isolation and Quantitation of beta-d-Glucopyranosyl Abscisate from Leaves of Xanthium and Spinach. Plant Physiol. 1982 Jul;70(1):227–231. doi: 10.1104/pp.70.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bray E. A., Zeevaart J. A. The Compartmentation of Abscisic Acid and beta-d-Glucopyranosyl Abscisate in Mesophyll Cells. Plant Physiol. 1985 Nov;79(3):719–722. doi: 10.1104/pp.79.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davenport T. L., Morgan P. W., Jordan W. R. Auxin Transport as Related to Leaf Abscission during Water Stress in Cotton. Plant Physiol. 1977 Apr;59(4):554–557. doi: 10.1104/pp.59.4.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davenport T. L., Morgan P. W., Jordan W. R. Reduction of auxin transport capacity with age and internal water deficits in cotton petioles. Plant Physiol. 1980 May;65(5):1023–1025. doi: 10.1104/pp.65.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guinn G., Brummett D. L., Beier R. C. Purification and measurement of abscisic Acid and indoleacetic Acid by high performance liquid chromatography. Plant Physiol. 1986 Aug;81(4):997–1002. doi: 10.1104/pp.81.4.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guinn G., Brummett D. L. Concentrations of abscisic Acid and indoleacetic Acid in cotton fruits and their abscission zones in relation to fruit retention. Plant Physiol. 1987 Jan;83(1):199–202. doi: 10.1104/pp.83.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guinn G. Water deficit and ethylene evolution by young cotton bolls. Plant Physiol. 1976 Mar;57(3):403–405. doi: 10.1104/pp.57.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewis L. N., Varner J. E. Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants. Plant Physiol. 1970 Aug;46(2):194–199. doi: 10.1104/pp.46.2.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ratner A., Goren R., Monselise S. P. Activity of pectin esterase and cellulase in the abscission zone of citrus leaf explants. Plant Physiol. 1969 Dec;44(12):1717–1723. doi: 10.1104/pp.44.12.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES