Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1988 Feb;86(2):332–334. doi: 10.1104/pp.86.2.332

Rapid Changes in Protein Phosphorylation Associated with Light-Induced Gravity Perception In Corn Roots 1

J J McFadden 1, B W Poovaiah 1
PMCID: PMC1054481  PMID: 11538234

Abstract

The effect of light and calcium depletion on in vivo protein phosphorylation was tested using dark-grown roots of Merit corn. Light caused rapid and specific promotion of phosphorylation of three polypeptides. Pretreatment of roots with ethylene glycol bis N,N,N′, N′ tetraacetic acid and A23187 prevented light-induced changes in protein phosphorylation. We postulate that these changes in protein phosphorylation are involved in the light-induced gravity response.

Full text

PDF
332

Images in this article

333Fig. 1
on p.333

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandurski R. S., Schulze A., Dayanandan P., Kaufman P. B. Response to gravity by Zea mays seedlings. I. Time course of the response. Plant Physiol. 1984;74:284–288. doi: 10.1104/pp.74.2.284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bensadoun A., Weinstein D. Assay of proteins in the presence of interfering materials. Anal Biochem. 1976 Jan;70(1):241–250. doi: 10.1016/s0003-2697(76)80064-4. [DOI] [PubMed] [Google Scholar]
  3. Cohen P. The role of protein phosphorylation in the hormonal control of enzyme activity. Eur J Biochem. 1985 Sep 16;151(3):439–448. doi: 10.1111/j.1432-1033.1985.tb09121.x. [DOI] [PubMed] [Google Scholar]
  4. Harmon A. C., Putnam-Evans C., Cormier M. J. A calcium-dependent but calmodulin-independent protein kinase from soybean. Plant Physiol. 1987 Apr;83(4):830–837. doi: 10.1104/pp.83.4.830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lee J. S., Mulkey T. J., Evans M. L. Gravity-Induced Polar Transport of Calcium across Root Tips of Maize. Plant Physiol. 1983 Dec;73(4):874–876. doi: 10.1104/pp.73.4.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lee J. S., Mulkey T. J., Evans M. L. Reversible loss of gravitropic sensitivity in maize roots after tip application of calcium chelators. Science. 1983 Jun 24;220(4604):1375–1376. doi: 10.1126/science.220.4604.1375. [DOI] [PubMed] [Google Scholar]
  7. Nelson A. J., Evans M. L. Analysis of growth patterns during gravitropic curvature in roots of Zea mays by use of a computer-based video digitizer. J Plant Growth Regul. 1986;5:73–83. doi: 10.1007/BF02025958. [DOI] [PubMed] [Google Scholar]
  8. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  9. Pickard B. G. Early events in geotropism of seedling shoots. Annu Rev Plant Physiol. 1985;36:55–75. doi: 10.1146/annurev.pp.36.060185.000415. [DOI] [PubMed] [Google Scholar]
  10. Poovaiah B. W., Reddy A. S. Calcium messenger system in plants. CRC Crit Rev Plant Sci. 1987;6(1):47–103. doi: 10.1080/07352688709382247. [DOI] [PubMed] [Google Scholar]
  11. Poovaiah B. W., Reddy A. S., McFadden J. J. Calcium messenger system: role of protein phosphorylation and inositol bisphospholipids. Physiol Plant. 1987;69:569–573. doi: 10.1111/j.1399-3054.1987.tb09241.x. [DOI] [PubMed] [Google Scholar]
  12. Raghothama K. G., Reddy A. S., Friedmann M., Poovaiah B. W. Calcium-regulated in vivo protein phosphorylation in Zea mays L. root tips. Plant Physiol. 1987;83:1008–1013. doi: 10.1104/pp.83.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reddy A. S., Chengappa S., Poovaiah B. W. Auxin-regulated changes in protein phosphorylation in pea epicotyls. Biochem Biophys Res Commun. 1987 Apr 29;144(2):944–950. doi: 10.1016/s0006-291x(87)80055-4. [DOI] [PubMed] [Google Scholar]
  14. Sack F. D., Suyemoto M. M., Leopold A. C. Amyloplast sedimentation kinetics in gravistimulated maize roots. Planta. 1985;165:295–300. [PubMed] [Google Scholar]
  15. Sibley D. R., Benovic J. L., Caron M. G., Lefkowitz R. J. Regulation of transmembrane signaling by receptor phosphorylation. Cell. 1987 Mar 27;48(6):913–922. doi: 10.1016/0092-8674(87)90700-8. [DOI] [PubMed] [Google Scholar]
  16. Veluthambi K., Poovaiah B. W. Calcium-promoted protein phosphorylation in plants. Science. 1984 Jan 13;223(4632):167–169. doi: 10.1126/science.223.4632.167. [DOI] [PubMed] [Google Scholar]
  17. Veluthambi K., Poovaiah B. W. In vitro and in vivo protein phosphorylation in Avena sativa L. coleoptiles: effects of Ca2+, calmodulin antagonists, and auxin. Plant Physiol. 1986 Jul;81(3):836–841. doi: 10.1104/pp.81.3.836. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES