Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1988 Feb;86(2):638–641. doi: 10.1104/pp.86.2.638

Characterization of Root Hair Cell Walls as Potential Barriers to the Infection of Plants by Rhizobia 1

The Carbohydrate Component

Andrew J Mort 1, Paul B Grover Jr 1,2
PMCID: PMC1054537  PMID: 16665960

Abstract

The sugar compositions of root hairs of a variety of plant species were determined. Root hairs of legumes had very similar compositions, whereas those of different families varied widely. Only approximately 50% of the weight of the root hairs of legumes could be accounted for by sugar. Up to 80% of the weight of root hairs from other sources could be accounted for by sugars. Protein made up 5 to 8% of the weight of root hairs of dicots but only 1.3% in corn. Comparison between cell walls from various root cell types within legumes showed that the polysaccharide compositions of root epidermal, root hair, and root cortical cells were very similar. Cotton root hairs were markedly different from walls of mesophyll and epidermal cells of cotyledons from cotton.

Full text

PDF
638

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhuvaneswari T. V., Turgeon B. G., Bauer W. D. Early Events in the Infection of Soybean (Glycine max L. Merr) by Rhizobium japonicum: I. LOCALIZATION OF INFECTIBLE ROOT CELLS. Plant Physiol. 1980 Dec;66(6):1027–1031. doi: 10.1104/pp.66.6.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaplin M. F. A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography. Anal Biochem. 1982 Jul 1;123(2):336–341. doi: 10.1016/0003-2697(82)90455-9. [DOI] [PubMed] [Google Scholar]
  3. Heinrikson R. L., Meredith S. C. Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. Anal Biochem. 1984 Jan;136(1):65–74. doi: 10.1016/0003-2697(84)90307-5. [DOI] [PubMed] [Google Scholar]
  4. Hubbell D. H., Morales V. M., Umali-Garcia M. Pectolytic enzymes in Rhizobium. Appl Environ Microbiol. 1978 Jan;35(1):210–213. doi: 10.1128/aem.35.1.210-213.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Martinez-Molina E., Morales V. M., Hubbell D. H. Hydrolytic enzyme production by Rhizobium. Appl Environ Microbiol. 1979 Dec;38(6):1186–1188. doi: 10.1128/aem.38.6.1186-1188.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McNeil M., Darvill A. G., Fry S. C., Albersheim P. Structure and function of the primary cell walls of plants. Annu Rev Biochem. 1984;53:625–663. doi: 10.1146/annurev.bi.53.070184.003205. [DOI] [PubMed] [Google Scholar]
  7. Mort A. J. An apparatus for safe and convenient handling of anhydrous, liquid hydrogen fluoride at controlled temperatures and reaction times. Application to the generation of oligosaccharides from polysaccharides. Carbohydr Res. 1983 Oct 28;122(2):315–321. doi: 10.1016/0008-6215(83)88342-6. [DOI] [PubMed] [Google Scholar]
  8. Mort A. J., Lamport D. T. Anhydrous hydrogen fluoride deglycosylates glycoproteins. Anal Biochem. 1977 Oct;82(2):289–309. doi: 10.1016/0003-2697(77)90165-8. [DOI] [PubMed] [Google Scholar]
  9. Talmadge K. W., Keegstra K., Bauer W. D., Albersheim P. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides. Plant Physiol. 1973 Jan;51(1):158–173. doi: 10.1104/pp.51.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES