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. 1988 Mar;86(3):822–825. doi: 10.1104/pp.86.3.822

A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis 1

Kai-Hsien Chen 1,2,3,2, Anita N Miller 1,2,3,3, Glenn W Patterson 1,2,3, Jerry D Cohen 1,2,3
PMCID: PMC1054577  PMID: 16665995

Abstract

A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.

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Selected References

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