Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1988 Mar;86(3):899–903. doi: 10.1104/pp.86.3.899

Early Activation by Ethylene of the Tonoplast H+-Pumping ATPase in the Latex from Hevea brasiliensis

Xavier Gidrol 1,2,1, Hervé Chrestin 1,2, Gilles Mounoury 1,2, Jean D'Auzac 1,2
PMCID: PMC1054591  PMID: 16666005

Abstract

The treatment of Hevea brasiliensis (rubber tree) bark by chloro-2-ethyl phosphonic acid (ethrel), an ethylene-producing compound, induces a significant increase in the tonoplast H+-translocating ATPase activity in the latex during the first 24 hours after the application of the stimulating agent. Moreover, the tonoplast-bound ATPase is highly activated when vacuoles (lutoids) are resuspended in ultrafiltrated cytosol. This effect is amplified during ethrel stimulation. Preliminary assays to characterize the endogenous effector(s) suggest that the activator(s) could be a heat-resistant compound with a low molecular weight, most likely an anion. The activation of the tonoplast-bound ATPase and the associated activation of the protons translocation across the lutoid membrane, could explain the cytosolic alkalinization observed in latex following the ethrel treatment of Hevea bark, which results in an enhanced rubber production.

Full text

PDF
899

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apelbaum A., Vinkler C., Sfakiotakis E., Dilley D. R. Increased mitochondrial DNA and RNA polymerase activity in ethylene-treated potato tubers. Plant Physiol. 1984 Oct;76(2):461–464. doi: 10.1104/pp.76.2.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chalutz E. Ethylene-induced Phenylalanine Ammonia-Lyase Activity in Carrot Roots. Plant Physiol. 1973 Jun;51(6):1033–1036. doi: 10.1104/pp.51.6.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christoffersen R. E., Laties G. G. Ethylene regulation of gene expression in carrots. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4060–4063. doi: 10.1073/pnas.79.13.4060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Churchill K. A., Sze H. Anion-Sensitive, H-Pumping ATPase of Oat Roots : Direct Effects of Cl, NO(3), and a Disulfonic Stilbene. Plant Physiol. 1984 Oct;76(2):490–497. doi: 10.1104/pp.76.2.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Lew R. R., Spanswick R. M. Characterization of Anion Effects on the Nitrate-Sensitive ATP-Dependent Proton Pumping Activity of Soybean (Glycine max L.) Seedling Root Microsomes. Plant Physiol. 1985 Feb;77(2):352–357. doi: 10.1104/pp.77.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lin W., Wagner G. J., Siegelman H. W., Hind G. Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta. 1977 Feb 14;465(1):110–117. doi: 10.1016/0005-2736(77)90359-5. [DOI] [PubMed] [Google Scholar]
  8. Marin B. P., Gidrol X. Chloride-ion stimulation of the tonoplast H+-translocating ATPase from Hevea brasiliensis (rubber tree) latex. A dual mechanism. Biochem J. 1985 Feb 15;226(1):85–94. doi: 10.1042/bj2260085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marin B., Gidrol X., Chrestin H., D'Auzac J. The tonoplast proton-translocating ATPase of higher plants as a third class of proton-pumps. Biochimie. 1986 Dec;68(12):1263–1277. doi: 10.1016/s0300-9084(86)80078-5. [DOI] [PubMed] [Google Scholar]
  10. Marin B., Marin-Lanza M., Komor E. The protonmotive potential difference across the vacuo-lysosomal membrane of Hevea brasiliensis (rubber tree) and its modification by a membrane-bound adenosine triphosphatase. Biochem J. 1981 Aug 15;198(2):365–372. doi: 10.1042/bj1980365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marin B., Preisser J., Komor E. Solubilization and purification of the ATPase from the tonoplast of Hevea. Eur J Biochem. 1985 Aug 15;151(1):131–140. doi: 10.1111/j.1432-1033.1985.tb09077.x. [DOI] [PubMed] [Google Scholar]
  12. Theologis A., Laties G. G. Potentiating effect of pure oxygen on the enhancement of respiration by ethylene in plant storage organs: a comparative study. Plant Physiol. 1982 May;69(5):1031–1035. doi: 10.1104/pp.69.5.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zurfluh L. L., Guilfoyle T. J. Auxin- and ethylene-induced changes in the population of translatable messenger RNA in Basal sections and intact soybean hypocotyl. Plant Physiol. 1982 Feb;69(2):338–340. doi: 10.1104/pp.69.2.338. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES