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. 1988 Apr;86(4):1127–1130. doi: 10.1104/pp.86.4.1127

31P-NMR Spectroscopy of Roots of Intact Corn Seedlings 1

Justin K M Roberts 1, M Pia Testa 1
PMCID: PMC1054639  PMID: 16666043

Abstract

The application of 31P nuclear magnetic resonance spectroscopy to the study of metabolism in roots of intact corn seedlings is described. 31P-NMR spectra of developmentally distinct parts of primary roots of whole seedlings are presented. The spectra are of quality comparable to those of excised pieces of plant tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrew E. R. The current status of NMR imaging. Biosci Rep. 1982 Sep;2(9):707–712. doi: 10.1007/BF01114833. [DOI] [PubMed] [Google Scholar]
  2. Bottomley P. A., Rogers H. H., Foster T. H. NMR imaging shows water distribution and transport in plant root systems in situ. Proc Natl Acad Sci U S A. 1986 Jan;83(1):87–89. doi: 10.1073/pnas.83.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Martin J. B., Bligny R., Rebeille F., Douce R., Leguay J. J., Mathieu Y., Guern J. A P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium. Plant Physiol. 1982 Oct;70(4):1156–1161. doi: 10.1104/pp.70.4.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pfeffer P. E., Tu S. I., Gerasimowicz W. V., Cavanaugh J. R. In VivoP NMR Studies of Corn Root Tissue and Its Uptake of Toxic Metals. Plant Physiol. 1986 Jan;80(1):77–84. doi: 10.1104/pp.80.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ricci D. R., Orlick A. E., Alderman E. L., Ingels N. B., Jr, Daughters G. T., 2nd, Stinson E. B. Influence of heart rate on left ventricular ejection fraction in human beings. Am J Cardiol. 1979 Sep;44(3):447–451. doi: 10.1016/0002-9149(79)90395-3. [DOI] [PubMed] [Google Scholar]
  6. Roberts J. K., Lane A. N., Clark R. A., Nieman R. H. Relationships between the rate of synthesis of ATP and the concentrations of reactants and products of ATP hydrolysis in maize root tips, determined by 31P nuclear magnetic resonance. Arch Biochem Biophys. 1985 Aug 1;240(2):712–722. doi: 10.1016/0003-9861(85)90080-3. [DOI] [PubMed] [Google Scholar]
  7. Roberts J. K., Linker C. S., Benoit A. G., Jardetzky O., Nieman R. H. Salt stimulation of phosphate uptake in maize root tips studied by p nuclear magnetic resonance. Plant Physiol. 1984 Aug;75(4):947–950. doi: 10.1104/pp.75.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roberts J. K., Wade-Jardetzky N., Jardetzky O. Intracellular pH measurements by 31P nuclear magnetic resonance. Influence of factors other than pH on 31P chemical shifts. Biochemistry. 1981 Sep 15;20(19):5389–5394. doi: 10.1021/bi00522a006. [DOI] [PubMed] [Google Scholar]
  9. Roberts J. K., Wemmer D., Jardetzky O. Measurement of mitochondrial ATPase activity in maize root tips by saturation transfer p nuclear magnetic resonance. Plant Physiol. 1984 Mar;74(3):632–639. doi: 10.1104/pp.74.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schaefer J., Stejskal E. O., Beard C. F. Carbon-13 Nuclear Magnetic Resonance Analysis of Metabolism in Soybean Labeled by CO(2). Plant Physiol. 1975 Jun;55(6):1048–1053. doi: 10.1104/pp.55.6.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]

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