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. 1988 Apr;86(4):1264–1269. doi: 10.1104/pp.86.4.1264

Auxin-Stimulated NADH Oxidase Purified from Plasma Membrane of Soybean 1

Andrew O Brightman 1,2, Rita Barr 1,2, Frederick L Crane 1,2, D James Morré 1,2
PMCID: PMC1054662  PMID: 16666065

Abstract

NADH oxidation by plasma membrane vesicles purified from hypocotyls of etiolated soybean seedlings by two-phase partition was stimulated 2- to 3-fold by auxins, indole-3-acetic acid, 2,4-dichlorophenoxy acetic acid (2,4-D), and α-naphthaleneacetic acid. The stimulation was concentration dependent in the presence or absence of detergent with a maximum for 2,4-D at 1 micromolar. The NADH oxidation activity was solubilized with the zwitterionic detergent CHAPS and purified by ion exchange chromatography and gel filtration approximately 2000-fold over the total homogenate. Both the partially purified fraction and an active band from nondenaturing gel electrophoresis revealed the same three bands when analyzed by denaturing gel electrophoresis. When obtained from plasma membrane vesicles from the region of rapid cell elongation, the NADH oxidase complex retained auxin responsiveness throughout purification (3- to 5-fold stimulation by 1 micromolar 2,4-D).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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