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. 1988 May;87(1):41–45. doi: 10.1104/pp.87.1.41

Purification and Characterization of a Specific Nucleoside Diphosphatase from Soybean Root Nodules 1

Holly D Doremus 1,2, Dale G Blevins 1
PMCID: PMC1054696  PMID: 16666123

Abstract

A specific nucleoside diphosphatase was purified from the plant portion of soybean (Glycine max L.) root nodules. This enzyme is highly specific for nucleotide diphosphates; it is unable to hydrolyze nucleotide tri- and monophosphates or a variety of other phosphorylated compounds. It will, however, hydrolyze any nucleotide disphosphate tested. The pH optimum of the enzyme is about 7.5; it requires a divalent cation for activity; and it is neither inhibited nor activated by any of the metabolites tested. It appears that in vivo this enzyme would be very active, but its function is not clear.

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Selected References

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  1. Dauwalder M., Whaley W. G., Kephart J. E. Phosphatases and differentiation of the Golgi apparatus. J Cell Sci. 1969 Mar;4(2):455–497. doi: 10.1242/jcs.4.2.455. [DOI] [PubMed] [Google Scholar]
  2. Doremus H. D., Blevins D. G. Nucleoside diphosphatase and 5'-nucleotidase activities of soybean root nodules and other tissues. Plant Physiol. 1988 May;87(1):36–40. doi: 10.1104/pp.87.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duggleby R. G. A nonlinear regression program for small computers. Anal Biochem. 1981 Jan 1;110(1):9–18. doi: 10.1016/0003-2697(81)90104-4. [DOI] [PubMed] [Google Scholar]
  4. GIBSON D. M., AYENGAR P., SANADI D. R. A phosphatase specific for nucleoside diphosphates. Biochim Biophys Acta. 1955 Apr;16(4):536–538. doi: 10.1016/0006-3002(55)90275-4. [DOI] [PubMed] [Google Scholar]
  5. Green J. R., Northcote D. H. Polyprenyl phosphate sugars synthesized during slime-polysaccharide production by membranes of the root-cap cells of maize (Zea mays). Biochem J. 1979 Mar 15;178(3):661–671. doi: 10.1042/bj1780661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hunt R. E., Haynes J. L., Cowles J. R. A possible new nucleoside diphosphatase from the cytosol of soybean and alfalfa nodules. Plant Physiol. 1977 Apr;59(4):773–774. doi: 10.1104/pp.59.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuriyama Y. Studies on microsomal nucleoside diphosphatase of rat hepatocytes. Its purification, intramembranous localization, and turnover. J Biol Chem. 1972 May 25;247(10):2979–2988. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lienhard G. E., Secemski I. I. P 1 ,P 5 -Di(adenosine-5')pentaphosphate, a potent multisubstrate inhibitor of adenylate kinase. J Biol Chem. 1973 Feb 10;248(3):1121–1123. [PubMed] [Google Scholar]
  10. Magnusson R. P., Portis A. R., Jr, McCarty R. E. Quantitative, analytical separation of adenine nucleotides by column chromatography on polyethyleneimine-coated cellulose. Anal Biochem. 1976 May 7;72:653–657. doi: 10.1016/0003-2697(76)90580-7. [DOI] [PubMed] [Google Scholar]
  11. Miggiano G. A., Mordente A., Martorana G. E., Meucci E., Castelli A. Characterization of alkaline phosphatase inactivation by ascorbic acid. Biochim Biophys Acta. 1984 Sep 25;789(3):343–346. doi: 10.1016/0167-4838(84)90190-0. [DOI] [PubMed] [Google Scholar]
  12. PLAUT G. W. An inosinediphosphatase from mammalian liver. J Biol Chem. 1955 Nov;217(1):235–245. [PubMed] [Google Scholar]
  13. Shinshi H., Miwa M., Kato K., Noguchi M., Matsushima T., Sugimura T. A novel phosphodiesterase from cultured tobacco cells. Biochemistry. 1976 May 18;15(10):2185–2190. doi: 10.1021/bi00655a024. [DOI] [PubMed] [Google Scholar]
  14. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
  15. Triplett E. W., Blevins D. G., Randall D. D. Allantoic Acid Synthesis in Soybean Root Nodule Cytosol via Xanthine Dehydrogenase. Plant Physiol. 1980 Jun;65(6):1203–1206. doi: 10.1104/pp.65.6.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]

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