Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1988 May;87(1):104–108. doi: 10.1104/pp.87.1.104

Evidence for a Na+/H+ Antiporter in Membrane Vesicles Isolated from Roots of the Halophyte Atriplex nummularia1

Yael Braun 1, Miriam Hassidim 1, Henri R Lerner 1, Leonora Reinhold 1
PMCID: PMC1054706  PMID: 16666082

Abstract

The ATP-dependent establishment of a positive membrane potential (measured as S14CN−-accumulation) in membrane vesicles isolated from the roots of Atriplex nummularia Lindl. was not inhibited by NaMes and KMes at concentrations up to 140 millimolar. On the other hand, the formation of ΔpH (measured as 14C-methylamine accumulation or quenching of quinacrine fluorescence), was depressed by NaMes concentrations as low as 30 millimolar. Supply of NaMes after the ΔpH had been established brought about partial dissipation within 30 seconds. Extent of dissipation of ΔpH increased with NaMes concentration over the range tested (up to 180 millimolar). The H+/Na+ exchange indicated by these results was not due to the creation of a Na+ diffusion potential. Formation of ΔpH in these vesicles was stable to NO3− up to 100 millimolar; further, the dissipating effect of Na+ supply was apparent on a ΔpH formed in the presence of 30 millimolar NO3−. Additional evidence that the origin of the membrane vesicles observed in this investigation was not the tonoplast and was probably the plasmalemma included the vanadate sensitivity of the establishment of the membrane potential.

Full text

PDF
104

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson P. S. Kinetic properties of the plasma membrane Na+-H+ exchanger. Annu Rev Physiol. 1985;47:545–560. doi: 10.1146/annurev.ph.47.030185.002553. [DOI] [PubMed] [Google Scholar]
  2. Blumwald E., Poole R. J. Na/H Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris. Plant Physiol. 1985 May;78(1):163–167. doi: 10.1104/pp.78.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Braun Y., Hassidim M., Lerner H. R., Reinhold L. Studies on H-Translocating ATPases in Plants of Varying Resistance to Salinity : I. Salinity during Growth Modulates the Proton Pump in the Halophyte Atriplex nummularia. Plant Physiol. 1986 Aug;81(4):1050–1056. doi: 10.1104/pp.81.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brey R. N., Rosen B. P., Sorensen E. N. Cation/proton antiport systems in Escherichia coli. Properties of the potassium/proton antiporter. J Biol Chem. 1980 Jan 10;255(1):39–44. [PubMed] [Google Scholar]
  6. Hassidim M., Braun Y., Lerner H. R., Reinhold L. Studies on H-Translocating ATPases in Plants of Varying Resistance to Salinity : II. K Strongly Promotes Development of Membrane Potential in Vesicles from Cotton Roots. Plant Physiol. 1986 Aug;81(4):1057–1061. doi: 10.1104/pp.81.4.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leonard R. T., Hansen D., Hodges T. K. Membrane-bound Adenosine Triphosphatase Activities of Oat Roots. Plant Physiol. 1973 Apr;51(4):749–754. doi: 10.1104/pp.51.4.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nakamura T., Hsu C., Rosen B. P. Cation/proton antiport systems in Escherichia coli. Solubilization and reconstitution of delta pH-driven sodium/proton and calcium/proton antiporters. J Biol Chem. 1986 Jan 15;261(2):678–683. [PubMed] [Google Scholar]
  9. O'neill S. D., Spanswick R. M. Effects of vanadate on the plasma membrane ATPase of red beet and corn. Plant Physiol. 1984 Jul;75(3):586–591. doi: 10.1104/pp.75.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Padan E., Zilberstein D., Schuldiner S. pH homeostasis in bacteria. Biochim Biophys Acta. 1981 Dec;650(2-3):151–166. doi: 10.1016/0304-4157(81)90004-6. [DOI] [PubMed] [Google Scholar]
  11. Schuldiner S., Fishkes H. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978 Feb 21;17(4):706–711. doi: 10.1021/bi00597a023. [DOI] [PubMed] [Google Scholar]
  12. Schumaker K. S., Sze H. A Ca/H Antiport System Driven by the Proton Electrochemical Gradient of a Tonoplast H-ATPase from Oat Roots. Plant Physiol. 1985 Dec;79(4):1111–1117. doi: 10.1104/pp.79.4.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Watad A. E., Pesci P. A., Reinhold L., Lerner H. R. Proton Fluxes as a Response to External Salinity in Wild Type and NaCl-Adapted Nicotiana Cell Lines. Plant Physiol. 1986 Jun;81(2):454–459. doi: 10.1104/pp.81.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. West I. C., Mitchell P. Proton/sodium ion antiport in Escherichia coli. Biochem J. 1974 Oct;144(1):87–90. doi: 10.1042/bj1440087. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES