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. 1988 May;87(1):217–222. doi: 10.1104/pp.87.1.217

Inositol-Containing Lipids in Suspension-Cultured Plant Cells

An Isotopic Study

Bjørn K Drøbak 1,2,3,4,1, Ian B Ferguson 1,2,3,4, Alan P Dawson 1,2,3,4, Robin F Irvine 1,2,3,4
PMCID: PMC1054728  PMID: 16666106

Abstract

Polar lipids were extracted from suspension-cultured tomato (Lycopersicon esculentum Mill.) cells and analyzed by thin layer chromatography. Four major inositol-containing compounds were found, and incorporation of [32P]orthosphosphate, [2-3H]glycerol, and myo-[2-3H]inositol was studied. Results showed that phosphatidylinositol-monophosphate is the phospholipid in these cells displaying the most rapid incorporation of [32P]orthophosphate. We suggest that the tracer is incorporated primarily into the phosphomonoester group. Two inositol-containing lipids showed chromatographic behavior similar to phosphatidylinositol-4,5-bisphosphate when using standard thin layer chromatography techniques. The labeling pattern of these compounds, however, reveals that it is unlikely that either of these is identical to phosphatidylinositol-4,5-bisphosphate. Should phosphatidylinositol-bisphosphate be present in suspension cultured plant cells, our data indicate chemical abundancies substantially lower than previously reported.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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