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. 1988 Aug;87(4):813–817. doi: 10.1104/pp.87.4.813

23Na-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina1

Michal Bental 1,2, Hadassa Degani 1,2, Mordhay Avron 1,2
PMCID: PMC1054851  PMID: 16666230

Abstract

The Intracellular Na+ concentration in the halotolerant alga Dunaliella salina was measured in intact cells by 23Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 ± 28 millimolar. Intracellular sodium ion content and intracellular volume were the same, within the experimental error, in cells adapted to grow in media containing between 0.1 and 4.0 molar NaCl. These values assume extracellular and intracellular NMR visibilities of the 23Na nuclei of 100 and 40%, respectively. The relaxation rate of intracellular sodium was enhanced with increasing salinity of the growth medium, in parallel to the intracellular osmosity due to the presence of glycerol, indicating that Na+ ions and glycerol are codistribbuted within the cell volume.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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