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. 1988 Sep;88(1):16–20. doi: 10.1104/pp.88.1.16

DNA Methylation Occurred around Lowly Expressed Genes of Plastid DNA during Tomato Fruit Development 1

Jarunya Ngernprasirtsiri 1,2,2, Hirokazu Kobayashi 1,2, Takashi Akazawa 1,2
PMCID: PMC1055517  PMID: 16666259

Abstract

We have analyzed DNA methylation of plastid DNA from fully ripened red fruits, green mature fruits, and green leaves of tomato (Lycopersicon esculentum var. Firstmore). Essentially identical restriction profiles were obtained between chromoplast and chloroplast DNAs by EcoRI digestion. BstNI/EcoRII and HpaII/MspI are pairs of isoschizomers that can discriminate between methylated and unmethylated DNAs. These endonucleases produced different restriction patterns of plastid DNAs from tomato fruits compared to tomato leaves. Moreover, we have found from Southern blots that methylation was not detected in DNA fragments containing certain genes that are actively expressed in chromoplasts, whereas DNA fragments bearing genes that are barely transcribed in chromoplasts are methylated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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