Abstract
Chromoplasts are a common differentiation state of plastids in which the photosynthetic apparatus is absent and carotenoids accumulate to high levels. As a first step toward the isolation of chromoplast-specific genes, we have examined plastids of the bell pepper, Capsicum annuum L., for the presence of chromoplast-specific proteins. Intact chromoplasts were isolated from mature fruits of C. annuum var Emerald Giant, Golden Cal Wonder, and DNAP VS-12 by differential centrifugation followed by isopycnic sedimentation in gradients of silica sols. The plastids were then fractionated into soluble and membrane components and the proteins analyzed by one- and two-dimensional gel electrophoresis using isoelectric focusing, sodium dodecyl sulfate, and sodium dodecyl sulfate-urea gels. Two polypeptides with Mr of 35,000 and 58,000 accumulate to high levels in membrane fractions of chromoplasts of var Emerald Giant. These polypeptides are either not detectable or barely detectable in chloroplasts from immature fruits. Both polypeptides have been purified to near homogeneity. Yellow chromoplasts from var Golden Cal Wonder and red chromoplasts from var DNAP VS-12 contained the 35-kilodalton polypeptide, but not the 58-kilodalton species.
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- Beyer P., Weiss G., Kleinig H. Solubilization and reconstitution of the membrane-bound carotenogenic enzymes from daffodil chromoplasts. Eur J Biochem. 1985 Dec 2;153(2):341–346. doi: 10.1111/j.1432-1033.1985.tb09308.x. [DOI] [PubMed] [Google Scholar]
- Camara B., Bardat F., Dogbo O., Brangeon J., Monéger R. Terpenoid Metabolism in Plastids : Isolation and Biochemical Characteristics of Capsicum annuum Chromoplasts. Plant Physiol. 1983 Sep;73(1):94–99. doi: 10.1104/pp.73.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREY-WYSSLING A., KREUTZER E. The submicroscopic development of chromoplasts in the fruit of Capsicum annuum L. J Ultrastruct Res. 1958 Aug;1(4):397–411. doi: 10.1016/s0022-5320(58)90010-8. [DOI] [PubMed] [Google Scholar]
- Giuliano G., Pollock D., Scolnik P. A. The gene crtI mediates the conversion of phytoene into colored carotenoids in Rhodopseudomonas capsulata. J Biol Chem. 1986 Oct 5;261(28):12925–12929. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Masamoto K., Riethman H. C., Sherman L. A. Isolation and Characterization of a Carotenoid-Associated Thylakoid Protein from the Cyanobacterium Anacystis nidulans R2. Plant Physiol. 1987 Jul;84(3):633–639. doi: 10.1104/pp.84.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piechulla B., Glick R. E., Bahl H., Melis A., Gruissem W. Changes in Photosynthetic Capacity and Photosynthetic Protein Pattern during Tomato Fruit Ripening. Plant Physiol. 1987 Jul;84(3):911–917. doi: 10.1104/pp.84.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao B. J., Brahmachari S. K., Rao M. R. Structural organization of the meiotic prophase chromatin in the rat testis. J Biol Chem. 1983 Nov 25;258(22):13478–13485. [PubMed] [Google Scholar]
- d'Harlingue A., Camara B. Plastid enzymes of terpenoid biosynthesis. Purification and characterization of gamma-tocopherol methyltransferase from Capsicum chromoplasts. J Biol Chem. 1985 Dec 5;260(28):15200–15203. [PubMed] [Google Scholar]