Abstract
Cytokinin oxidase activity from Phaseolus vulgaris cv Great Northern callus cultures exhibited affinity for the lectin concanavalin A. Over 80% of the activity extracted from the callus tissue bound to a concanavalin A-Sepharose 4B column. The bound activity was eluted from the column by the addition of methylmannose to the eluting buffer. On the basis of this result, it appears that most of the cyokinin oxidase activity present in Great Northern callus cultures exists in the form of a glycoprotein. The apparent pI of this enzyme, as estimated by chromatofocusing, is approximately 5.0.
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- Brownlee B. G., Hall R. H., Whitty C. D. 3-Methyl-2-butenal: an enzymatic degradation product of the cytokinin, N-6-(delta-2 isopentenyl)adenine. Can J Biochem. 1975 Jan;53(1):37–41. doi: 10.1139/o75-006. [DOI] [PubMed] [Google Scholar]
- Chatfield J. M., Armstrong D. J. Cytokinin Oxidase from Phaseolus vulgaris Callus Tissues : Enhanced in Vitro Activity of the Enzyme in the Presence of Copper-Imidazole Complexes. Plant Physiol. 1987 Jul;84(3):726–731. doi: 10.1104/pp.84.3.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatfield J. M., Armstrong D. J. Regulation of Cytokinin Oxidase Activity in Callus Tissues of Phaseolus vulgaris L. cv Great Northern. Plant Physiol. 1986 Feb;80(2):493–499. doi: 10.1104/pp.80.2.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paces V., Kamínek M. Effect of ribosylzeatin isomers on the enzymatic degradation of N6-(delta2-isopentenyl) adenosine. Nucleic Acids Res. 1976 Sep;3(9):2309–2314. doi: 10.1093/nar/3.9.2309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paces V., Werstiuk E., Hall R. H. Conversion of N-(Delta-Isopentenyl)adenosine to Adenosine by Enzyme Activity in Tobacco Tissue. Plant Physiol. 1971 Dec;48(6):775–778. doi: 10.1104/pp.48.6.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whitty C. D., Hall R. H. A cytokinin oxidase in Zea mays. Can J Biochem. 1974 Sep;52(9):789–799. doi: 10.1139/o74-112. [DOI] [PubMed] [Google Scholar]