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. 1988 Oct;88(2):370–374. doi: 10.1104/pp.88.2.370

Regulation of Gene Expression by Ethylene in Wild-Type and rin Tomato (Lycopersicon esculentum) Fruit 1

James E Lincoln 1, Robert L Fischer 1
PMCID: PMC1055583  PMID: 16666310

Abstract

Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increase the rate of ethylene biosynthesis at the onset of ripening, rin fruit constitutively produce ethylene at a low basal level. We have compared the mRNA levels and transcription rates of four cloned ethylene-inducible genes (JE Lincoln, S Cordes, E Read, RL Fischer 1987 Proc Natl Acad Sci USA 84: 2793-2797) during wild-type and rin fruit development. In wild-type fruit, both mRNA levels and transcription rates of these genes increase. The effect of the rin mutation on gene expression is different for each ethylene-inducible gene. In one case expression is completely suppressed, while in other instances it is either partially inhibited or relatively unaffected by the mutation. The mRNA levels of each of these genes in response to exogenous ethylene in rin fruit was also measured. The mRNAs for all four genes accumulate to similar levels in both ethylene treated rin and ethylene treated wild-type fruit. These results are discussed with regard to the response of plants to ethylene hormone at the level of gene expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dellapenna D., Kates D. S., Bennett A. B. Polygalacturonase Gene Expression in Rutgers, rin, nor, and Nr Tomato Fruits. Plant Physiol. 1987 Oct;85(2):502–507. doi: 10.1104/pp.85.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Herner R. C., Sink K. C. Ethylene Production and Respiratory Behavior of the rin Tomato Mutant. Plant Physiol. 1973 Jul;52(1):38–42. doi: 10.1104/pp.52.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hofer E., Darnell J. E., Jr The primary transcription unit of the mouse beta-major globin gene. Cell. 1981 Feb;23(2):585–593. doi: 10.1016/0092-8674(81)90154-9. [DOI] [PubMed] [Google Scholar]
  4. Hofer E., Hofer-Warbinek R., Darnell J. E., Jr Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure. Cell. 1982 Jul;29(3):887–893. doi: 10.1016/0092-8674(82)90450-0. [DOI] [PubMed] [Google Scholar]
  5. Kende H., Hanson A. D. Relationship between Ethylene Evolution and Senescence in Morning-Glory Flower Tissue. Plant Physiol. 1976 Apr;57(4):523–527. doi: 10.1104/pp.57.4.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lincoln J. E., Cordes S., Read E., Fischer R. L. Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc Natl Acad Sci U S A. 1987 May;84(9):2793–2797. doi: 10.1073/pnas.84.9.2793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lincoln J. E., Fischer R. L. Diverse mechanisms for the regulation of ethylene-inducible gene expression. Mol Gen Genet. 1988 Apr;212(1):71–75. doi: 10.1007/BF00322446. [DOI] [PubMed] [Google Scholar]
  8. McGlasson W. B., Dostal H. C., Tigchelaar E. C. Comparison of Propylene-induced Responses of Immature Fruit of Normal and rin Mutant Tomatoes. Plant Physiol. 1975 Feb;55(2):218–222. doi: 10.1104/pp.55.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGlasson W. B., Poovaiah B. W., Dostal H. C. Ethylene production and respiration in aging leaf segments and in disks of fruit tissue of normal and mutant tomatoes. Plant Physiol. 1975 Oct;56(4):547–549. doi: 10.1104/pp.56.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Thomas P. S. Hybridization of denatured RNA transferred or dotted nitrocellulose paper. Methods Enzymol. 1983;100:255–266. doi: 10.1016/0076-6879(83)00060-9. [DOI] [PubMed] [Google Scholar]
  12. Walling L., Drews G. N., Goldberg R. B. Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123–2127. doi: 10.1073/pnas.83.7.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]

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