Abstract
A laser-based photoacoustic method was used for determination of ethylene (C2H4) production of emasculated orchid (Cymbidium) flowers in a flow-through system. The laser photoacoustic equipment consisted of a line-tuneable CO2 laser in conjunction with a single-pass resonant acoustic cell. The minimum detection limit of the system for C2H4 in air was 0.03 nanoliter per liter. C2H4 production of intact Cymbidium (cv Mary Pinchess `Del Rey') flowers was very low (0.015 nanoliter per gram per hour) and showed an increase within 3 hours following emasculation (removal of pollinia plus anthercap). Production peaked (0.14 nanoliter per gram per hour) 8 hours after emasculation and decreased thereafter. Production again increased 45 hours after emasculation. Coloration of the labellum appeared shortly after the first peak; wilting of the petals and sepals appeared during the second rise in ethylene production. The use of the laser photoacoustic technique in plant physiological studies is discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bassi P. K., Spencer M. S. A Cuvette Design for Measurement of Ethylene Production and Carbon Dioxide Exchange by Intact Shoots under Controlled Environmental Conditions. Plant Physiol. 1979 Sep;64(3):488–490. doi: 10.1104/pp.64.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bassi P. K., Spencer M. S. Does light inhibit ethylene production in leaves? Plant Physiol. 1983 Nov;73(3):758–760. doi: 10.1104/pp.73.3.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burg S. P., Dijkman M. J. Ethylene and auxin participation in pollen induced fading of vanda orchid blossoms. Plant Physiol. 1967 Nov;42(11):1648–1650. doi: 10.1104/pp.42.11.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dhawan K. R., Bassi P. K., Spencer M. S. Effects of carbon dioxide on ethylene production and action in intact sunflower plants. Plant Physiol. 1981 Oct;68(4):831–834. doi: 10.1104/pp.68.4.831. [DOI] [PMC free article] [PubMed] [Google Scholar]