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. 1988 Nov;88(3):780–784. doi: 10.1104/pp.88.3.780

Quantification of Indole-3-Acetic Acid in Dark-Grown Seedlings of the Diageotropica and Epinastic Mutants of Tomato (Lycopersicon esculentum Mill.) 1

David W Fujino 1,2,3,4, Scott J Nissen 1,2,3,4, A Daniel Jones 1,2,3,4, David W Burger 1,2,3,4, Kent J Bradford 1,2,3,4
PMCID: PMC1055660  PMID: 16666383

Abstract

Endogenous indoleacetic acid (IAA) levels were examined in 7-day-old, dark-grown tomato seedlings (Lycopersicon esculentum Mill. cv VFN8), and in two single-gene mutants, Epinastic and diageotropica. Gas chromatography-mass spectrometry was employed to quantify IAA using 13C6-[benzene ring]indoleacetic acid as internal standard. IAA concentrations ranged from 89 to 134 nanograms per gram dry weight and were not significantly different for the three genotypes. Ethylene over-production by dark-grown Epi seedlings is not likely to result from increased IAA. Assuming similar recovery percentages for each genotype, indole-3-ethanol, a purported storage form of IAA, was identified by GC-MS and found to be more prevalent in the parent tomato, VFN8, with only trace amounts observed in Epi. No IEt was detected by high performance liquid chromatography/fluorescence in dgt (detection limit >100 picograms).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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