Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1988 Nov;88(3):943–946. doi: 10.1104/pp.88.3.943

Immunocytolocalization of Glutamine Synthetase in Green Leaves and Cotyledons of Lycopersicon esculentum1

Jose R Botella 1,2, Jean P Verbelen 1,2, Victoriano Valpuesta 1,2
PMCID: PMC1055686  PMID: 16666408

Abstract

Glutamine synthetase was localized in leaves and cotyledons of young tomato (Lycopersicon esculentum Mill.) plants using immunogold techniques coupled to transmission electron microscopy. The enzyme occurs only in chloroplasts and is most probably a stroma constituent.

Full text

PDF
943

Images in this article

945Fig. 5
on p.945
945Fig. 6
on p.945
945Fig. 7
on p.945
945Fig. 8
on p.945
944Fig. 1
on p.944
944Fig. 2
on p.944
944Fig. 3
on p.944
944Fig. 4
on p.944

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botella J. R., Canovas F. M., Avila C., de Castro I. N., Valpuesta V. Immunochemical comparison of glutamine synthetases from some solanaceae plants. Plant Physiol. 1986 Oct;82(2):585–587. doi: 10.1104/pp.82.2.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Botella J. R., Verbelen J. P., Valpuesta V. Immunocytolocalization of Ferredoxin-GOGAT in the Cells of Green Leaves and Cotyledons of Lycopersicon esculentum. Plant Physiol. 1988 May;87(1):255–257. doi: 10.1104/pp.87.1.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hirel B., McNally S. F., Gadal P., Sumar N., Stewart G. R. Cytosolic glutamine synthetase in higher plants. A comparative immunological study. Eur J Biochem. 1984 Jan 2;138(1):63–66. doi: 10.1111/j.1432-1033.1984.tb07881.x. [DOI] [PubMed] [Google Scholar]
  4. Hirel B., Perrot-Rechenmann C., Suzuki A., Vidal J., Gadal P. Glutamine Synthetase in Spinach Leaves : IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION. Plant Physiol. 1982 Apr;69(4):983–987. doi: 10.1104/pp.69.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mann A. F., Fentem P. A., Stewart G. R. Tissue localization of barley (Hordeum vulgare) glutamine synthetase isoenzymes. FEBS Lett. 1980 Feb 11;110(2):265–267. doi: 10.1016/0014-5793(80)80088-3. [DOI] [PubMed] [Google Scholar]
  6. McNally S. F., Hirel B., Gadal P., Mann A. F., Stewart G. R. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf. Plant Physiol. 1983 May;72(1):22–25. doi: 10.1104/pp.72.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Somerville C. R., Ogren W. L. Photorespiration-deficient Mutants of Arabidopsis thaliana Lacking Mitochondrial Serine Transhydroxymethylase Activity. Plant Physiol. 1981 Apr;67(4):666–671. doi: 10.1104/pp.67.4.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wallsgrove R. M., Lea P. J., Miflin B. J. Distribution of the Enzymes of Nitrogen Assimilation within the Pea Leaf Cell. Plant Physiol. 1979 Feb;63(2):232–236. doi: 10.1104/pp.63.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. van Tilbeurgh H., Pettersson G., Bhikabhai R., De Boeck H., Claeyssens M. Studies of the cellulolytic system of Trichoderma reesei QM 9414. Reaction specificity and thermodynamics of interactions of small substrates and ligands with the 1,4-beta-glucan cellobiohydrolase II. Eur J Biochem. 1985 Apr 15;148(2):329–334. doi: 10.1111/j.1432-1033.1985.tb08843.x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES