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. 1988 Dec;88(4):961–964. doi: 10.1104/pp.88.4.961

Characterization of Elemental Sulfur in Isolated Intact Spinach Chloroplasts

Jacques Joyard 1,2,3, Eric Forest 1,2,3, Elizabeth Blée 1,2,3, Roland Douce 1,2,3
PMCID: PMC1055693  PMID: 16666485

Abstract

Incubation of intact spinach (Spinacia oleracea L.) chloroplasts in the presence of 35SO42− resulted in the light-dependent formation of a chloroform-soluble sulfur-containing compound distinct from sulfolipid. We have identified this compound as the most stable form (S8) of elemental sulfur (S0, valence state for S = O) by mass spectrometry. It is possible that elemental sulfur (S0) was formed by oxidation of bound sulfide, i.e. after the photoreduction of sulfate to sulfide by intact chloroplasts, and released as S8 under the experimental conditions used for analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BENSON A. A., MARUO B. Piant phospholipids. I. Identification of the phosphatidyl glycerols. Biochim Biophys Acta. 1958 Jan;27(1):189–195. doi: 10.1016/0006-3002(58)90308-1. [DOI] [PubMed] [Google Scholar]
  3. BENSON A. A. THE PLANT SULFOLIPID. Adv Lipid Res. 1963;1:387–394. doi: 10.1016/b978-1-4831-9937-5.50016-8. [DOI] [PubMed] [Google Scholar]
  4. Garlick S., Oren A., Padan E. Occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria. J Bacteriol. 1977 Feb;129(2):623–629. doi: 10.1128/jb.129.2.623-629.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hageage G. J., Jr, Eanes E. D., Gherna R. L. X-ray diffraction studies of the sulfur globules accumulated by Chromatium species. J Bacteriol. 1970 Feb;101(2):464–469. doi: 10.1128/jb.101.2.464-469.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hajra A. K. On extraction of acyl and alkyl dihydroxyacetone phosphate from incubation mixtures. Lipids. 1974 Aug;9(8):502–505. doi: 10.1007/BF02532495. [DOI] [PubMed] [Google Scholar]
  7. Hansen T. A., van Gemerden H. Sulfide utilization by purple nonsulfur bacteria. Arch Mikrobiol. 1972;86(1):49–56. doi: 10.1007/BF00412399. [DOI] [PubMed] [Google Scholar]
  8. Kleppinger-Sparace K. F., Mudd J. B., Bishop D. G. Biosynthesis of sulfoquinovosyldiacylglycerol in higher plants: the incorporation of 35SO4 by intact chloroplasts. Arch Biochem Biophys. 1985 Aug 1;240(2):859–865. doi: 10.1016/0003-9861(85)90096-7. [DOI] [PubMed] [Google Scholar]
  9. Krauss F., Schäfer W., Schmidt A. Formation of Elemental Sulfur by Chlorella fusca during Growth on l-Cysteine Ethylester. Plant Physiol. 1984 Jan;74(1):176–182. doi: 10.1104/pp.74.1.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Legris-Delaporte S., Ferron F., Landry J., Costes C. Metabolization of elemental sulfur in wheat leaves consecutive to its foliar application. Plant Physiol. 1987 Dec;85(4):1026–1030. doi: 10.1104/pp.85.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moriarty D. J., Nicholas D. J. Products of sulphide oxidation in extracts of Thiobacillus concretivorus. Biochim Biophys Acta. 1970 Mar 3;197(2):143–151. doi: 10.1016/0005-2728(70)90025-3. [DOI] [PubMed] [Google Scholar]
  12. Mourioux G., Douce R. Transport du sulfate à travers la double membrane limitante, ou enveloppe, des chloroplastes d'épinard. Biochimie. 1979;61(11-12):1283–1292. [PubMed] [Google Scholar]
  13. Pezet R., Pont V. Elemental sulfur: accumulation in different species of fungi. Science. 1977 Apr 22;196(4288):428–429. doi: 10.1126/science.850786. [DOI] [PubMed] [Google Scholar]
  14. Truper H. G., Hathaway J. C. Orthorhombic sulphur formed by photosynthetic sulphur bacteria. Nature. 1967 Jul 22;215(5099):435–436. doi: 10.1038/215435a0. [DOI] [PubMed] [Google Scholar]

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