Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1988 Dec;88(4):987–989. doi: 10.1104/pp.88.4.987

Measurements of Proton Pumping in Arum maculatum Mitochondria

Betty K Ishida 1,2, John M Palmer 1,2
PMCID: PMC1055700  PMID: 16666492

Abstract

Direct evidence is presented for the lack of a pH change in the medium associated with electron flow along the alternative, cyanide-resistant pathway in Arum maculatum mitochondria. When these mitochondria oxidize succinate via the cytochrome chain, the medium becomes acidified. However, when the main respiratory chain is blocked by cyanide or antimycin A, oxygen consumption continues, but no pH change occurs. This confirms the lack of a charge separation across the membrane, so that phosphorylation cannot be supported during operation of the alternative oxidase.

Full text

PDF
987

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Doussière J., Sainsard-Chanet A., Vignais P. V. The respiratory chain of Paramecium tetraurelia in wild type and the mutant Cl1. II. Cyanide-insensitive respiration. Function and regulation. Biochim Biophys Acta. 1979 Nov 8;548(2):236–252. doi: 10.1016/0005-2728(79)90132-4. [DOI] [PubMed] [Google Scholar]
  2. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  3. Mitchell P., Moyle J. Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria. Eur J Biochem. 1969 Feb;7(4):471–484. doi: 10.1111/j.1432-1033.1969.tb19633.x. [DOI] [PubMed] [Google Scholar]
  4. Moore A. L., Bonner W. D., Jr, Rich P. R. The determination of the proton-motive force during cyanide-insensitive respiration in plant mitochondria. Arch Biochem Biophys. 1978 Mar;186(2):298–306. doi: 10.1016/0003-9861(78)90439-3. [DOI] [PubMed] [Google Scholar]
  5. Moore A. L., Bonner W. D. Measurements of membrane potentials in plant mitochondria with the safranine method. Plant Physiol. 1982 Nov;70(5):1271–1276. doi: 10.1104/pp.70.5.1271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Palmer J. M., Kirk B. I. The influence of osmolarity on the reduction of exogenous cytochrome c and permeability of the inner membrane of Jerusalem artichoke mitochondria. Biochem J. 1974 Apr;140(1):79–86. doi: 10.1042/bj1400079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schonbaum G. R., Bonner W. D., Jr, Storey B. T., Bahr J. T. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids. Plant Physiol. 1971 Jan;47(1):124–128. doi: 10.1104/pp.47.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Slater E. C. Mechanism of oxidative phosphorylation. Annu Rev Biochem. 1977;46:1015–1026. doi: 10.1146/annurev.bi.46.070177.005055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wilson S. B. Energy conservation by the plant mitochondrial cyanide-insensitive oxidase. Some additional evidence. Biochem J. 1980 Aug 15;190(2):349–360. doi: 10.1042/bj1900349. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES