Abstract
Enzymically active cell wall from ripe tomato (Lycopersicon esculentum Mill.) fruit pericarp release uronic acids through the action of wall-bound polygalacturonase. The potential involvement of products of wall hydrolysis in the induction of ethylene synthesis during tomato ripening was investigated by vacuum infiltrating preclimacteric (green) fruit with solutions containing pectin fragments enzymically released from cell wall from ripe fruit. Ripening initiation was accelerated in pectin-infiltrated fruit compared to control (buffer-infiltrated) fruit as measured by initiation of climacteric CO2 and ethylene production and appearance of red color. The response to infiltration was maximum at a concentration of 25 micrograms pectin per fruit; higher concentrations (up to 125 micrograms per fruit) had no additional effect. When products released from isolated cell wall from ripe pericarp were separated on Bio-Gel P-2 and specific size classes infiltrated into preclimacteric fruit, ripening-promotive activity was found only in the larger (degree of polymerization >8) fragments. Products released from pectin derived from preclimacteric pericarp upon treatment with polygalacturonase from ripe pericarp did not stimulate ripening when infiltrated into preclimacteric fruit.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
- Felsenfeld G. DNA. Sci Am. 1985 Oct;253(4):58–67. doi: 10.1038/scientificamerican1085-58. [DOI] [PubMed] [Google Scholar]
- Hahn M. G., Darvill A. G., Albersheim P. Host-Pathogen Interactions : XIX. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS. Plant Physiol. 1981 Nov;68(5):1161–1169. doi: 10.1104/pp.68.5.1161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobson G. E., Richardson C., Gillham D. J. Release of protein from normal and mutant tomato cell walls. Plant Physiol. 1983 Mar;71(3):635–638. doi: 10.1104/pp.71.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huber D. J., Lee J. H. Uronic Acid products release from enzymically active cell wall from tomato fruit and its dependency on enzyme quantity and distribution. Plant Physiol. 1988 Jul;87(3):592–597. doi: 10.1104/pp.87.3.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kim J., Gross K. C., Solomos T. Characterization of the Stimulation of Ethylene Production by Galactose in Tomato (Lycopersicon esculentum Mill.) Fruit. Plant Physiol. 1987 Nov;85(3):804–807. doi: 10.1104/pp.85.3.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poovaiah B. W., Nukaya A. Polygalacturonase and Cellulase Enzymes in the Normal Rutgers and Mutant rin Tomato Fruits and Their Relationship to the Respiratory Climacteric. Plant Physiol. 1979 Oct;64(4):534–537. doi: 10.1104/pp.64.4.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rushing J. W., Huber D. J. In vitro characterization of tomato fruit softening : the use of enzymically active cell walls. Plant Physiol. 1984 Aug;75(4):891–894. doi: 10.1104/pp.75.4.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tong C. B., Labavitch J. M., Yang S. F. The induction of ethylene production from pear cell culture by cell wall fragments. Plant Physiol. 1986 Jul;81(3):929–930. doi: 10.1104/pp.81.3.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tucker G. A., Robertson N. G., Grierson D. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit. Eur J Biochem. 1980 Nov;112(1):119–124. doi: 10.1111/j.1432-1033.1980.tb04993.x. [DOI] [PubMed] [Google Scholar]