Abstract
Russet spotting (RS) is a physiological disorder induced in iceberg lettuce (Lactuca sativa L.) by exposure to parts per million levels of ethylene at 5 ± 2°C. Ethylene induced phenylalanine ammonia-lyase and ionically bound peroxidase activities that correlated with development of RS symptoms. The ethylene-treated tissue had significantly higher lignin content than air control tissue with lignification localized in walls of RS-affected cells. Ethylene also caused the accumulation of the flavonoids (+)catechin and (−)epicatechin and the chlorogenic acid derivatives 3-caffeoyl-quinic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. These soluble phenolic compounds were readily oxidized to brown substances by polyphenol oxidase isolated from RS tissue. Ethylene substantially increased ionically bound indole-3-acetic acid (IAA) oxidase activity, while IAA application greatly reduced ethylene-induced phenylalanine ammonia-lyase, peroxidase, and IAA oxidase activities, soluble phenolic content, and RS development.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hyodo H., Kuroda H., Yang S. F. Induction of phenylalanine ammonia-lyase and increase in phenolics in lettuce leaves in relation to the development of russet spotting caused by ethylene. Plant Physiol. 1978 Jul;62(1):31–35. doi: 10.1104/pp.62.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mäder M., Amberg-Fisher V. Role of peroxidase in lignification of tobacco cells : I. Oxidation of nicotinamide adenine dinucleotide and formation of hydrogen peroxide by cell wall peroxidases. Plant Physiol. 1982 Oct;70(4):1128–1131. doi: 10.1104/pp.70.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mäder M., Füssl R. Role of Peroxidase in Lignification of Tobacco Cells : II. Regulation by Phenolic Compounds. Plant Physiol. 1982 Oct;70(4):1132–1134. doi: 10.1104/pp.70.4.1132. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sequeira L., Mineo L. Partial purification and kinetics of indoleacetic Acid oxidase from tobacco roots. Plant Physiol. 1966 Sep;41(7):1200–1208. doi: 10.1104/pp.41.7.1200. [DOI] [PMC free article] [PubMed] [Google Scholar]