Abstract
The accumulation of radiolabeled plastid-encoded chlorophyll a-apoproteins is light dependent and is controlled at a posttranscriptional level. Illumination of dark-grown barley (Hordeum vulgare L.) with a brief pulse of red light induced the accumulation of radiolabeled chlorophyll a-apoproteins in subsequent protein synthesis assays. The induction of radiolabeled chlorophyll a-apoprotein accumulation was not affected by pretreatment of leaves with cycloheximide. Fluence response studies showed that a red light photoreceptor controls the accumulation of radiolabeled chlorophyll a-apoproteins with a threshold fluence of approximately 50 to 100 microeinsteins per square meter. While red light initiated chlorophyll a-apoprotein accumulation, this process was not reversed by a far red light treatment given immediately after the pulse of red light. The light pulse which initiated the accumulation of radiolabeled chlorophyll a-apoproteins also induced the rapid conversion of protochlorophyllide to chlorophyll a. A chlorophyll-deficient mutant, xan-f10, which is blocked in chlorophyll biosynthesis prior to protochlorophyllide formation, failed to accumulate radiolabeled chlorophyll a-apoproteins in the light even though transcripts for these apoproteins were present. A second mutant, xan-j64, which accumulates chlorophyllide in the light but only low levels of chlorophyll a, also showed reduced accumulation of radiolabeled chlorophyll a-apoproteins upon illumination. These results suggest that the light-induced conversion of protochlorophyllide to chlorophyll a is necessary for accumulation of the plastid-encoded chlorophyll a-apoproteins and one red light photoreceptor controlling this response is the protochlorophyllide holochrome.
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- Apel K. The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Phytochrome-induced decrease of translatable mRNA coding for the NADPH: protochlorophyllide oxidoreductase. Eur J Biochem. 1981 Nov;120(1):89–93. doi: 10.1111/j.1432-1033.1981.tb05673.x. [DOI] [PubMed] [Google Scholar]
- Batschauer A., Mösinger E., Kreuz K., Dörr I., Apel K. The implication of a plastid-derived factor in the transcriptional control of nuclear genes encoding the light-harvesting chlorophyll a/b protein. Eur J Biochem. 1986 Feb 3;154(3):625–634. doi: 10.1111/j.1432-1033.1986.tb09444.x. [DOI] [PubMed] [Google Scholar]
- Fluhr R., Kuhlemeier C., Nagy F., Chua N. H. Organ-specific and light-induced expression of plant genes. Science. 1986 May 30;232(4754):1106–1112. doi: 10.1126/science.232.4754.1106. [DOI] [PubMed] [Google Scholar]
- Gounaris K., Barber J., Harwood J. L. The thylakoid membranes of higher plant chloroplasts. Biochem J. 1986 Jul 15;237(2):313–326. doi: 10.1042/bj2370313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horwitz B. A., Thompson W. F., Briggs W. R. Phytochrome Regulation of Greening in Pisum: Chlorophyll Accumulation and Abundance of mRNA for the Light-Harvesting Chlorophyll a/b Binding Proteins. Plant Physiol. 1988 Jan;86(1):299–305. doi: 10.1104/pp.86.1.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Roberts L. L., Briggs W. R., Thompson W. F. Phytochrome Control of Specific mRNA levels in Developing Pea Buds : Kinetics of Accumulation, Reciprocity, and Escape Kinetics of the Low Fluence Response. Plant Physiol. 1986 Aug;81(4):1033–1038. doi: 10.1104/pp.81.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufman L. S., Thompson W. F., Briggs W. R. Different Red Light Requirements for Phytochrome-Induced Accumulation of cab RNA and rbcS RNA. Science. 1984 Dec 21;226(4681):1447–1449. doi: 10.1126/science.226.4681.1447. [DOI] [PubMed] [Google Scholar]
- Klein R. R., Mason H. S., Mullet J. E. Light-regulated translation of chloroplast proteins. I. Transcripts of psaA-psaB, psbA, and rbcL are associated with polysomes in dark-grown and illuminated barley seedlings. J Cell Biol. 1988 Feb;106(2):289–301. doi: 10.1083/jcb.106.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein R. R., Mullet J. E. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings. J Biol Chem. 1987 Mar 25;262(9):4341–4348. [PubMed] [Google Scholar]
- Klein R. R., Mullet J. E. Regulation of chloroplast-encoded chlorophyll-binding protein translation during higher plant chloroplast biogenesis. J Biol Chem. 1986 Aug 25;261(24):11138–11145. [PubMed] [Google Scholar]
- Mullet J. E., Klein R. R., Grossman A. R. Optimization of protein synthesis in isolated higher plant chloroplasts. Identification of paused translation intermediates. Eur J Biochem. 1986 Mar 3;155(2):331–338. doi: 10.1111/j.1432-1033.1986.tb09495.x. [DOI] [PubMed] [Google Scholar]
- Mösinger E., Batschauer A., Apel K., Schäfer E., Briggs W. R. Phytochrome regulation of greening in barley : effects on mRNA abundance and on transcriptional activity of isolated nuclei. Plant Physiol. 1988 Mar;86(3):706–710. doi: 10.1104/pp.86.3.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Santel H. J., Apel K. The protochlorophyllide holochrome of barley (Hordeum vulgare L.). The effect of light on the NADPH:protochlorophyllide oxidoreductase. Eur J Biochem. 1981 Nov;120(1):95–103. doi: 10.1111/j.1432-1033.1981.tb05674.x. [DOI] [PubMed] [Google Scholar]
- Schiff J. A. A green safelight for the study of chloroplast development and other photomorphogenetic. Methods Enzymol. 1972;24:321–322. doi: 10.1016/0076-6879(72)24079-4. [DOI] [PubMed] [Google Scholar]
- Zhu Y. S., Kung S. D., Bogorad L. Phytochrome control of levels of mRNA complementary to plastid and nuclear genes of maize. Plant Physiol. 1985 Oct;79(2):371–376. doi: 10.1104/pp.79.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]