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. 1989 Jan;89(1):15–18. doi: 10.1104/pp.89.1.15

Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors 1

M Goldschmidt-Clermont 1,2, P Malnoë 1,2,2, J-D Rochaix 1,2
PMCID: PMC1055791  PMID: 16666506

Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.

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Selected References

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