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. 1989 Jan;89(1):204–210. doi: 10.1104/pp.89.1.204

Enhancement of Cyanobacterial Salt Tolerance by Combined Nitrogen 1

Bontha R Reddy 1, Shree K Apte 1, Joseph Thomas 1,2
PMCID: PMC1055820  PMID: 16666516

Abstract

Presence of certain nitrogenous compounds in the growth medium significantly enhanced the salt tolerance of the fresh-water cyanobacterium Anabaena sp. strain L-31 as well as the brackish water cyanobacterium Anabaena torulosa. Among these, nitrate, ammonium, and glutamine were most effective followed by glutamate and aspartate. These nitrogenous compounds also inhibited Na+ influx in both Anabaena spp. with the same order of effectiveness as that observed for protection against salt stress. The inhibition of Na+ influx on addition of the nitrogenous substances was rapid; nitrate and ammonium inhibited Na+ influx competitively. Proline and glycine did not affect Na+ influx and also had no influence on the salt tolerance of either Anabaena sp. The observed protection was not consequent to a stimulatory effect of combined nitrogen on growth per se. Uptake of NO3− and NH4+ increased during salt stress but was not correlated with growth. Intracellular levels of NO3− and NH4+ were found to be inadequate to constitute a major component of the internal osmoticum. The results suggest that inhibition of Na+ influx by combined nitrogen is a major mechanism for protection of cyanobacteria against salt stress.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apte S. K., Reddy B. R., Thomas J. Relationship between Sodium Influx and Salt Tolerance of Nitrogen-Fixing Cyanobacteria. Appl Environ Microbiol. 1987 Aug;53(8):1934–1939. doi: 10.1128/aem.53.8.1934-1939.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Apte S. K., Thomas J. Membrane electrogenesis and sodium transport in filamentous nitrogen-fixing cyanobacteria. Eur J Biochem. 1986 Jan 15;154(2):395–401. doi: 10.1111/j.1432-1033.1986.tb09411.x. [DOI] [PubMed] [Google Scholar]
  3. Blumwald E., Mehlhorn R. J., Packer L. Studies of osmoregulation in salt adaptation of cyanobacteria with ESR spin-probe techniques. Proc Natl Acad Sci U S A. 1983 May;80(9):2599–2602. doi: 10.1073/pnas.80.9.2599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumwald E., Tel-Or E. Salt adaptation of the cyanobacterium synechococcus 6311 growing in a continuous culture (turbidostat). Plant Physiol. 1984 Jan;74(1):183–185. doi: 10.1104/pp.74.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cheeseman C. I., Devlin D. The effect of amino acids and dipeptides on sodium-ion transport in rat enterocytes. Biochim Biophys Acta. 1985 Feb 14;812(3):767–773. doi: 10.1016/0005-2736(85)90270-6. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Le Rudulier D., Strom A. R., Dandekar A. M., Smith L. T., Valentine R. C. Molecular biology of osmoregulation. Science. 1984 Jun 8;224(4653):1064–1068. doi: 10.1126/science.224.4653.1064. [DOI] [PubMed] [Google Scholar]
  8. Miller D. M., Jones J. H., Yopp J. H., Tindall D. R., Schmid W. E. Ion metabolism in a halophilic blue-green alga, Aphanothece halophytica. Arch Microbiol. 1976 Dec 1;111(1-2):145–149. doi: 10.1007/BF00446561. [DOI] [PubMed] [Google Scholar]
  10. Paschinger H. DCCD induced sodium uptake by Anacystis nidulans. Arch Microbiol. 1977 Jun 20;113(3):285–291. doi: 10.1007/BF00492037. [DOI] [PubMed] [Google Scholar]
  11. Rottenberg H. The measurement of membrane potential and deltapH in cells, organelles, and vesicles. Methods Enzymol. 1979;55:547–569. doi: 10.1016/0076-6879(79)55066-6. [DOI] [PubMed] [Google Scholar]
  12. Sprott G. D., Shaw K. M., Jarrell K. F. Ammonia/potassium exchange in methanogenic bacteria. J Biol Chem. 1984 Oct 25;259(20):12602–12608. [PubMed] [Google Scholar]
  13. Suput D. Effect of external ammonium on the kinetics of the sodium current in frog muscle. Biochim Biophys Acta. 1984 Mar 28;771(1):1–8. doi: 10.1016/0005-2736(84)90102-0. [DOI] [PubMed] [Google Scholar]
  14. Thomas J. Absence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga. Nature. 1970 Oct 10;228(5267):181–183. doi: 10.1038/228181b0. [DOI] [PubMed] [Google Scholar]
  15. Weissmann J., Taborda de Messias I. J., Pribilla O. Vergleichende Untersuchungen über den Properdin-Faktor B (Bf)-Polymorphismus an Stichproben aus Brasilien, Deutschland und Guinea-Bissau. Anthropol Anz. 1983 Dec;41(4):301–307. [PubMed] [Google Scholar]
  16. Wolk C. P., Thomas J., Shaffer P. W., Austin S. M., Galonsky A. Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica. J Biol Chem. 1976 Aug 25;251(16):5027–5034. [PubMed] [Google Scholar]

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