Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1989 Jan;89(1):360–367. doi: 10.1104/pp.89.1.360

Effect on in Vitro Pollen Growth of an Isolated Style Glycoprotein Associated with Self-Incompatibility in Nicotiana alata1

Philip J Harris 1,2, Jodie A Weinhandl 1, Adrienne E Clarke 1
PMCID: PMC1055844  PMID: 16666539

Abstract

The effect on in vitro pollen tube growth of an isolated style glycoprotein (S2-glycoprotein) associated with self-incompatibility in Nicotiana alata was investigated. Tube growth of pollen bearing the S2-allele was inhibited, but tube growth of pollen bearing other alleles was not affected. Inhibition showed a dose response effect. The percentage of pollen grains that germinated was not significantly affected by the S2-glycoprotein. Growth of S2-pollen in the presence of the S2-glycoprotein resulted in increased binding to the pollen of monoclonal antibody (PCBC3) which has a primary specificity for α-l-arabinofuranosyl residues. Growth of pollen bearing other alleles in the presence of the glycoprotein resulted in no increased binding of the antibody.

Full text

PDF
360

Images in this article

362Figure 2
on p.362

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. A., Sandrin M. S., Clarke A. E. A high proportion of hybridomas raised to a plant extract secrete antibody to arabinose or galactose. Plant Physiol. 1984 Aug;75(4):1013–1016. doi: 10.1104/pp.75.4.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Clarke A. E., Anderson M. A., Bacic T., Harris P. J., Mau S. L. Molecular basis of cell recognition during fertilization in higher plants. J Cell Sci Suppl. 1985;2:261–285. doi: 10.1242/jcs.1985.supplement_2.14. [DOI] [PubMed] [Google Scholar]
  4. Ferrari T. E., Bruns D., Wallace D. H. Isolation of a plant glycoprotein involved with control of intercellular recognition. Plant Physiol. 1981 Feb;67(2):270–277. doi: 10.1104/pp.67.2.270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harris P. J., Freed K., Anderson M. A., Weinhandl J. A., Clarke A. E. An enzyme-linked immunosorbent assay (ELISA) for in vitro pollen growth based on binding of a monoclonal antibody to the pollen tube surface. Plant Physiol. 1987 Jul;84(3):851–855. doi: 10.1104/pp.84.3.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nishio T., Hinata K. Comparative Studies on S-Glycoproteins Purified from Different S-Genotypes in Self-Incompatible BRASSICA Species I. Purification and Chemical Properties. Genetics. 1982 Apr;100(4):641–647. doi: 10.1093/genetics/100.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES