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. 1989 Apr;89(4):1238–1246. doi: 10.1104/pp.89.4.1238

In Vivo31P NMR Spectroscopic Studies of Soybean Bradyrhizobium Symbiosis

I. Optimization of Parameters

Dominique B Rolin 1,2, Richard T Boswell 1,2, Charles Sloger 1,2, Shu-I Tu 1,2, Philip E Pfeffer 1,2
PMCID: PMC1056002  PMID: 16666690

Abstract

31P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the perfusion medium affected the cytoplasmic pH and the resolution of the spectra. The internal Pi content and distribution were assessed as a function of nodule age and green-house growth conditions and the rate of glucose and 2-deoxyglucose uptake into nodules in split and intact states. The major metabolites (glucose-6-P, fructose-1,6-diP, P-choline, Pi, NTP, UDP-glc, and NAD) were readily identified from 31P NMR spectra of perchloric acid extracts of nodules with the exception of one unknown phosphorus metabolite. Nodules stressed by glucose deprivation demonstrated movement of Pi between the vacuole and cytoplasmic compartments not previously observed in 31P NMR studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Elgavish A., Elgavish G. A., Halmann M., Berman T., Shomer I. Intracellular phosphorus pools in intact algal cells: 31P NMR and transmission electron microscopy studies. FEBS Lett. 1980 Aug 11;117(1):137–142. doi: 10.1016/0014-5793(80)80930-6. [DOI] [PubMed] [Google Scholar]
  2. Jackson P. C., Pfeffer P. E., Gerasimowicz W. V. Use of P NMR to Assess Effects of DNP on ATP Levels in Vivo in Barley Roots. Plant Physiol. 1986 Aug;81(4):1130–1133. doi: 10.1104/pp.81.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Martin J. B., Bligny R., Rebeille F., Douce R., Leguay J. J., Mathieu Y., Guern J. A P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium. Plant Physiol. 1982 Oct;70(4):1156–1161. doi: 10.1104/pp.70.4.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pfeffer P. E., Tu S. I., Gerasimowicz W. V., Cavanaugh J. R. In VivoP NMR Studies of Corn Root Tissue and Its Uptake of Toxic Metals. Plant Physiol. 1986 Jan;80(1):77–84. doi: 10.1104/pp.80.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rebeille F., Bligny R., Martin J. B., Douce R. Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch Biochem Biophys. 1983 Aug;225(1):143–148. doi: 10.1016/0003-9861(83)90017-6. [DOI] [PubMed] [Google Scholar]
  6. Roberts J. K., Callis J., Jardetzky O., Walbot V., Freeling M. Cytoplasmic acidosis as a determinant of flooding intolerance in plants. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6029–6033. doi: 10.1073/pnas.81.19.6029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Roberts J. K., Lane A. N., Clark R. A., Nieman R. H. Relationships between the rate of synthesis of ATP and the concentrations of reactants and products of ATP hydrolysis in maize root tips, determined by 31P nuclear magnetic resonance. Arch Biochem Biophys. 1985 Aug 1;240(2):712–722. doi: 10.1016/0003-9861(85)90080-3. [DOI] [PubMed] [Google Scholar]
  8. Roberts J. K., Linker C. S., Benoit A. G., Jardetzky O., Nieman R. H. Salt stimulation of phosphate uptake in maize root tips studied by p nuclear magnetic resonance. Plant Physiol. 1984 Aug;75(4):947–950. doi: 10.1104/pp.75.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roberts J. K., Wemmer D., Jardetzky O. Measurement of mitochondrial ATPase activity in maize root tips by saturation transfer p nuclear magnetic resonance. Plant Physiol. 1984 Mar;74(3):632–639. doi: 10.1104/pp.74.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roby C., Martin J. B., Bligny R., Douce R. Biochemical changes during sucrose deprivation in higher plant cells. Phosphorus-31 nuclear magnetic resonance studies. J Biol Chem. 1987 Apr 15;262(11):5000–5007. [PubMed] [Google Scholar]
  11. Streeter J. G. Accumulation of alpha,alpha-trehalose by Rhizobium bacteria and bacteroids. J Bacteriol. 1985 Oct;164(1):78–84. doi: 10.1128/jb.164.1.78-84.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. den Hollander J. A., Ugurbil K., Brown T. R., Shulman R. G. Phosphorus-31 nuclear magnetic resonance studies of the effect of oxygen upon glycolysis in yeast. Biochemistry. 1981 Sep 29;20(20):5871–5880. doi: 10.1021/bi00523a034. [DOI] [PubMed] [Google Scholar]
  13. van Berkum P., Sloger C. Immediate acetylene reduction by excised grass roots not previously preincubated at low oxygen tensions. Plant Physiol. 1979 Nov;64(5):739–743. doi: 10.1104/pp.64.5.739. [DOI] [PMC free article] [PubMed] [Google Scholar]

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