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. 1989 Apr;89(4):1351–1357. doi: 10.1104/pp.89.4.1351

Biochemical and Histochemical Localization of Monoterpene Biosynthesis in the Glandular Trichomes of Spearmint (Mentha spicata) 1,2

Jonathan Gershenzon 1, Massimo Maffei 1,3, Rodney Croteau 1
PMCID: PMC1056021  PMID: 16666709

Abstract

The primary monoterpene accumulated in the glandular trichomes of spearmint (Mentha spicata) is the ketone (−)-carvone which is formed by cyclization of the C10 isoprenoid intermediate geranyl pyrophosphate to the olefin (−)-limonene, hydroxylation to (−)-trans-carveol and subsequent dehydrogenation. Selective extraction of the contents of the glandular trichomes indicated that essentially all of the cyclase and hydroxylase activities resided in these structures, whereas only about 30% of the carveol dehydrogenase was located here with the remainder located in the rest of the leaf. This distribution of carveol dehydrogenase activity was confirmed by histochemical methods. Electrophoretic analysis of the partially purified carveol dehydrogenase from extracts of both the glands and the leaves following gland removal indicated the presence of a unique carveol dehydrogenase species in the glandular trichomes, suggesting that the other dehydrogenase found throughout the leaf probably utilizes carveol only as an adventitious substrate. These results demonstrate that carvone biosynthesis takes place exclusively in the glandular trichomes in which this natural product accumulates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Brewer J. M. Artifact produced in disc electrophoresis by ammonium persulfate. Science. 1967 Apr 14;156(3772):256–257. doi: 10.1126/science.156.3772.256. [DOI] [PubMed] [Google Scholar]
  3. Croteau R., Felton M., Karp F., Kjonaas R. Relationship of Camphor Biosynthesis to Leaf Development in Sage (Salvia officinalis). Plant Physiol. 1981 Apr;67(4):820–824. doi: 10.1104/pp.67.4.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Croteau R., Martinkus C. Metabolism of Monoterpenes: Demonstration of (+)-Neomenthyl-beta-d-Glucoside as a Major Metabolite of (-)-Menthone in Peppermint (Mentha Piperita). Plant Physiol. 1979 Aug;64(2):169–175. doi: 10.1104/pp.64.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Croteau R., Venkatachalam K. V. Metabolism of monoterpenes: demonstration that (+)-cis-isopulegone, not piperitenone, is the key intermediate in the conversion of (-)-isopiperitenone to (+)-pulegone in peppermint (Mentha piperita). Arch Biochem Biophys. 1986 Sep;249(2):306–315. doi: 10.1016/0003-9861(86)90007-x. [DOI] [PubMed] [Google Scholar]
  6. Croteau R., Winters J. N. Demonstration of the Intercellular Compartmentation of l-Menthone Metabolism in Peppermint (Mentha piperita) Leaves. Plant Physiol. 1982 Apr;69(4):975–977. doi: 10.1104/pp.69.4.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Daddow L. Y. A double lead stain method for enhancing contrast of ultrathin sections in electron microscopy: a modified multiple staining technique. J Microsc. 1983 Feb;129(Pt 2):147–153. doi: 10.1111/j.1365-2818.1983.tb04169.x. [DOI] [PubMed] [Google Scholar]
  8. Davisson V. J., Woodside A. B., Poulter C. D. Synthesis of allylic and homoallylic isoprenoid pyrophosphates. Methods Enzymol. 1985;110:130–144. doi: 10.1016/s0076-6879(85)10068-6. [DOI] [PubMed] [Google Scholar]
  9. Dehal S. S., Croteau R. Metabolism of monoterpenes: specificity of the dehydrogenases responsible for the biosynthesis of camphor, 3-thujone, and 3-isothujone. Arch Biochem Biophys. 1987 Oct;258(1):287–291. doi: 10.1016/0003-9861(87)90346-8. [DOI] [PubMed] [Google Scholar]
  10. Gershenzon J., Duffy M. A., Karp F., Croteau R. Mechanized techniques for the selective extraction of enzymes from plant epidermal glands. Anal Biochem. 1987 May 15;163(1):159–164. doi: 10.1016/0003-2697(87)90106-0. [DOI] [PubMed] [Google Scholar]
  11. Heeb M. J., Gabriel O. Enzyme localization in gels. Methods Enzymol. 1984;104:416–439. doi: 10.1016/s0076-6879(84)04109-4. [DOI] [PubMed] [Google Scholar]
  12. Keene C. K., Wagner G. J. Direct demonstration of duvatrienediol biosynthesis in glandular heads of tobacco trichomes. Plant Physiol. 1985 Dec;79(4):1026–1032. doi: 10.1104/pp.79.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kjonaas R. B., Venkatachalam K. V., Croteau R. Metabolism of monoterpenes: oxidation of isopiperitenol to isopiperitenone, and subsequent isomerization to piperitenone by soluble enzyme preparations from peppermint (Mentha piperita) leaves. Arch Biochem Biophys. 1985 Apr;238(1):49–60. doi: 10.1016/0003-9861(85)90139-0. [DOI] [PubMed] [Google Scholar]
  14. Kjonaas R., Croteau R. Demonstration that limonene is the first cyclic intermediate in the biosynthesis of oxygenated p-menthane monoterpenes in Mentha piperita and other Mentha species. Arch Biochem Biophys. 1983 Jan;220(1):79–89. doi: 10.1016/0003-9861(83)90389-2. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]

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