REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
rabbit anti-cFOS	Synaptic Systems	Cat#226003; RRID: 2231974
goat anti-rabbit Alexa Fluor 488	Thermo Fisher	Cat#A11034; RRID: AB_2576217
rabbit anti-DsRed	Takara Bio Clontech	Cat#632496; RRID: AB_10013483
Bacterial and virus strains
AAV2-flex-taCasp3-TEVp	UNC Vector Core (Depositor: Nirao Shah & Jim Wells) Yang et al.58
AAV8-hSyn-hM3D(Gq)-mCherry	Addgene (Depositor: Brian Roth) Krashes et al.57	Addgene viral prep Cat#50474-AAV8
AAV2-FLEX-tdTomato	Addgene (Depositor: Edward Boyden)	Addgene plasmid Cat#28306
AAV8-Syn-FLEX-Mac-GFP	Addgene (Depositor: Edward Boyden)	Addgene plasmid Cat#58852
Chemicals, peptides, and recombinant proteins
Clozapine-n-oxide (CNO)	Millipore Sigma	Cat#0832
Giemsa	Fisher	Cat#G146-10
DIG RNA labeling kit	Roche	SKU#11277073910
Fluorescein RNA Labeling Mix	Roche	SKU#11685619910
RNA Clean & Concentrator	Zymo Research	Cat#R1017
ZymoPURE II Plasmid Midiprep kit	Zymo Research	Cat#D4201
Zymo DNA Clean & Concentrator	Zymo Research	Cat#D4033
Blocking Reagent	Roche	Cat#11096176001
TSA Plus Cyanine 5 System	Akoya Biosciences	Cat#NEL745001KT
Rneasy Micro kit	Qiagen	Cat#74004
Deposited data
RNA seq data from neurons FACS isolated from mouse tuberal hypothalamus neurons	NCI Gene Expression Omnibus (GEO)	GSE224254
GTEx	Broad Institute	Version 8
Molecular Signatures Database	Broad Institute	Human Gene Set: HALLMARK_ESTROGEN_RESPONSE_EARLY
Datasets for estrogen signaling binning of human data	Github	https://github.com/Leandromvelez/sex-specific-endocrine-signals.
Experimental models: Organisms/strains
model organism: Sst-Cre: genotype STOCK Ssttm2.1(cre)Zjh/J	The Jackson Laboratory	Strain#013044
model organism: Esr1-Cre: genotype B6N.129S6(Cg)-Esr1tm1.1(cre)And/J	The Jackson Laboratory	Strain#017911
Recombinant DNA
cDNA for Sst (bases 7-550 for transcript variant 1, bases 317-860 for transcript variant 2)	Allen Brain Atlas	Probe: RP_Baylor_103041
TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli	Invitrogen	Cat#K203001
Software and algorithms
VitalView Software	Starr Life Sciences	version 5.1
lme() function (nlme package, version 3.1-157)	r-project.org	R version 4.2.1
t_test() function (rstatix package, version 0.7.0)	r-project.org	R version 4.2.1
Code for volcano plot	Github	http://github.com/jevanveen/ratplots R function: deseq_volcano_plot_gs()
Analysis pipleline for estrogen signaling binning of human data and cross-tissue correlations with mouse orthologues	Github	https://github.com/Leandromvelez/sex-specific-endocrine-signals
Kallisto	Pachter Lab Bray et al.114	version 0.46.2
Deseq2	Galaxy	Version 2.11.40.6+galaxy1
CellProfiler	Broad Institute	4.2.1