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. 1986 Oct;82(2):411–416. doi: 10.1104/pp.82.2.411

Identification of Granule-Bound Starch Synthase in Potato Tubers 1

Greetje H Vos-Scheperkeuter 1,2, Wietse de Boer 1,2, Richard G F Visser 1,2, Will J Feenstra 1,2, Bernard Witholt 1,2
PMCID: PMC1056132  PMID: 16665043

Abstract

Starch granules isolated from potato (Solanum tuberosum L.) tubers were extracted with sodium dodecyl sulfate and the extract was analyzed. A major protein with a molecular weight of 60,000 daltons was detected. This protein was purified by preparative sodium dodecyl sulfate-gel electrophoresis and specific antibodies were prepared. The anti-60-kilodalton antibodies obtained (a) cross-reacted with the waxy proteins of both maize (Zea mays L.) and grain amaranth (Amaranthus hypochondriacus L.), and (b) inhibited starch synthase activity in partially digested starch granules of the grain amaranth. This evidence strongly suggests that the major 60-kilodalton protein present in potato starch granules represents the granule-bound starch synthase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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