Abstract
N-Hydroxyacylnornicotine, newly discovered from fresh plant tissue, was found entirely in the trichome exudate produced at the epidermis of the aerial part of Nicotiana stocktonii. Nicotine and nornicotine, but not N-hydroxyacylnornicotine, were present inside of the trichomes as well as other internal parts of the plant. Only nicotine was found in bleeding sap squeezed from cut roots or stems. Feeding of leaves with 2′-14C-labeled nicotine primarily yielded labeled nicotine, nornicotine, and N-hydroxyacylnornicotine. When similarly labeled nornicotine was fed to leaves as a precursor, a labeled N-hydroxyacylnornicotine was obtained, with a higher specific activity than with the [2′-14C]nicotine feeding. Based on these results, a synthesis route is suggested where nicotine is converted in the leaf to nornicotine, followed by trichome conversion of nornicotine to N-hydroxyacylnornicotine, and rapid secretion of this product.
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- Alworth W. L., Rapoport H. Biosynthesis of the nicotine alkaloids in Nicotiana glutinosa. Interrelationships among nicotine, nornicotine, anabasine, and anatabine. Arch Biochem Biophys. 1965 Oct;112(1):45–53. doi: 10.1016/0003-9861(65)90008-1. [DOI] [PubMed] [Google Scholar]
- Keene C. K., Wagner G. J. Direct demonstration of duvatrienediol biosynthesis in glandular heads of tobacco trichomes. Plant Physiol. 1985 Dec;79(4):1026–1032. doi: 10.1104/pp.79.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUMPF P. K., JAMES A. T. The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations. Biochim Biophys Acta. 1963 Feb 19;70:20–32. doi: 10.1016/0006-3002(63)90715-7. [DOI] [PubMed] [Google Scholar]