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. 1986 Oct;82(2):585–587. doi: 10.1104/pp.82.2.585

Immunochemical Comparison of Glutamine Synthetases from Some Solanaceae Plants 1

Jose R Botella 1, Francisco M Canovas 1, Concepcion Avila 1, Ignacio Nuñez de Castro 1, Victoriano Valpuesta 1
PMCID: PMC1056162  PMID: 16665071

Abstract

The presence of different glutamine synthetase isoenzymes in different Solanaceae plants and their relative antigenicities against antiglutamine synthetase from tomato leaf serum were studied. All the plants tested showed one glutamine synthetase isoenzyme except for Mandragora autumnalis, which showed two, after discontinuous polyacrylamide gel electrophoresis and specific in situ assay. Antigenicities were compared by the double immunodiffusion technique. The Nicotiana glauca enzyme showed equal reactivity to that of Lycopersicon esculentum, but its antigenicity was higher than Withania frutescens, Datura stramonium, and Hyoscyamus niger. The study of relative antigenicities permitted differentiation of the glutamine synthetase enzymes from uncultivated species of Solanaceae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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