Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1986 Oct;82(2):610–612. doi: 10.1104/pp.82.2.610

High CO2 Requiring Mutant of Anacystis nidulans R21

Yehouda Marcus 1,2, Rakefet Schwarz 1,2, Devorah Friedberg 1,2, Aaron Kaplan 1,2
PMCID: PMC1056170  PMID: 16665079

Abstract

Some physiological characteristics of a mutant (E1) of Anacystis nidulans R2, incapable of growing at air level of CO2, are described. E1 is capable of accumulating inorganic carbon (Ci) internally as efficiently as the wild type (R2). The apparent photosynthetic affinity for Ci in E1, however, is some 1000 times lower than that of R2. The kinetic parameters of ribulose 1,5-bisphosphate carboxylase/oxygenase from E1 are similar to those observed in R2. The mutant appears to be defective in its ability to utilize the intracellular Ci pool for photosynthesis and depends on extracellular supply of Ci in the form of CO2. The very high apparent photosynthetic Km (CO2) of the mutant indicate a large diffusion resistance for CO2. Data obtained here are used to calculate the permeability coefficient for CO2 between the bulk medium and the carboxylation site of cyanobacteria.

Full text

PDF
610

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger M. R., Bassett M., Comins H. N. A Model for HCO(3) Accumulation and Photosynthesis in the Cyanobacterium Synechococcus sp: Theoretical Predictions and Experimental Observations. Plant Physiol. 1985 Feb;77(2):465–471. doi: 10.1104/pp.77.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Badger M. R., Kaplan A., Berry J. A. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep;66(3):407–413. doi: 10.1104/pp.66.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Badger M. R. Kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase from Anabaena variabilis. Arch Biochem Biophys. 1980 Apr 15;201(1):247–254. doi: 10.1016/0003-9861(80)90509-3. [DOI] [PubMed] [Google Scholar]
  4. Herdman M., Carr N. G. The isolation and characterization of mutant strains of the blue-green alga anacystis nidulans. J Gen Microbiol. 1972 Apr;70(2):213–220. doi: 10.1099/00221287-70-2-213. [DOI] [PubMed] [Google Scholar]
  5. Marcus Y., Harel E., Kaplan A. Adaptation of the Cyanobacterium Anabaena variabilis to Low CO(2) Concentration in Their Environment. Plant Physiol. 1983 Jan;71(1):208–210. doi: 10.1104/pp.71.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marcus Y., Zenvirth D., Harel E., Kaplan A. Induction of HCO(3) Transporting Capability and High Photosynthetic Affinity to Inorganic Carbon by Low Concentration of CO(2) in Anabaena variabilis. Plant Physiol. 1982 May;69(5):1008–1012. doi: 10.1104/pp.69.5.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller A. G., Colman B. Active transport and accumulation of bicarbonate by a unicellular cyanobacterium. J Bacteriol. 1980 Sep;143(3):1253–1259. doi: 10.1128/jb.143.3.1253-1259.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Simon S. A., Gutknecht J. Solubility of carbon dioxide in lipid bilayer membranes and organic solvents. Biochim Biophys Acta. 1980 Mar 13;596(3):352–358. doi: 10.1016/0005-2736(80)90122-4. [DOI] [PubMed] [Google Scholar]
  9. Spalding M. H., Spreitzer R. J., Ogren W. L. Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth. Plant Physiol. 1983 Oct;73(2):268–272. doi: 10.1104/pp.73.2.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Volokita M., Zenvirth D., Kaplan A., Reinhold L. Nature of the Inorganic Carbon Species Actively Taken Up by the Cyanobacterium Anabaena variabilis. Plant Physiol. 1984 Nov;76(3):599–602. doi: 10.1104/pp.76.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES