Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1986 Nov;82(3):671–674. doi: 10.1104/pp.82.3.671

ATPase in Lipid Body Membranes of Castor Bean Endosperm 1

Matthew J Hills 1, Harry Beevers 1
PMCID: PMC1056186  PMID: 16665089

Abstract

Lipid body membranes purified from castor seed endosperm of dry seeds and 4 d old seedlings were found to have an ATPase activity associated with them. This was confirmed by equilibrium density centrifugation of the membranes using acid lipase as a marker enzyme. The specific activity ranged from 45 to 200 nanomoles per milligram protein per minute. The pH optimum was 9.0 but at pH 7.5 nearly 40% of the maximum activity was retained. The apparent Km for Mg-ATP was 0.5 millimolar. A divalent cation was required for activity and Mg2+ was the most effective. Other nucleoside triphosphates were also hydrolyzed but there was no hydrolysis of pyrophosphate or p-nitrophenylphosphate. The ATPase was not inhibited by oligomycin, vanadate, dicyclohexylcarbodiimide, or molybdate but was inhibited by sodium azide. Washing the membranes with increasing concentrations of NaCl removed up to 60% of the ATPase activity but none was removed by 3 millimolar ethylene-diaminetetraacetate.

Full text

PDF
671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balke N. E., Hodges T. K. Plasma membrane adenosine triphosphatase of oat roots: activation and inhibition by mg and ATP. Plant Physiol. 1975 Jan;55(1):83–86. doi: 10.1104/pp.55.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chanson A., Taiz L. Evidence for an ATP-Dependent Proton Pump on the Golgi of Corn Coleoptiles. Plant Physiol. 1985 Jun;78(2):232–240. doi: 10.1104/pp.78.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fujiki Y., Hubbard A. L., Fowler S., Lazarow P. B. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol. 1982 Apr;93(1):97–102. doi: 10.1083/jcb.93.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lanzetta P. A., Alvarez L. J., Reinach P. S., Candia O. A. An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem. 1979 Nov 15;100(1):95–97. doi: 10.1016/0003-2697(79)90115-5. [DOI] [PubMed] [Google Scholar]
  6. Lin Y. H., Wimer L. T., Huang A. H. Lipase in the Lipid Bodies of Corn Scutella during Seedling Growth. Plant Physiol. 1983 Oct;73(2):460–463. doi: 10.1104/pp.73.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maeshima M., Beevers H. Purification and properties of glyoxysomal lipase from castor bean. Plant Physiol. 1985 Oct;79(2):489–493. doi: 10.1104/pp.79.2.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moreau R. A., Liu K. D., Huang A. H. Spherosomes of Castor Bean Endosperm: MEMBRANE COMPONENTS, FORMATION, AND DEGRADATION. Plant Physiol. 1980 Jun;65(6):1176–1180. doi: 10.1104/pp.65.6.1176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Muto S., Beevers H. Lipase Activities in Castor Bean Endosperm during Germination. Plant Physiol. 1974 Jul;54(1):23–28. doi: 10.1104/pp.54.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  11. Rungie J. M., Wiskich J. T. Salt-stimulated Adenosine Triphosphatase from Smooth Microsomes of Turnip. Plant Physiol. 1973 Jun;51(6):1064–1068. doi: 10.1104/pp.51.6.1064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yatsu L. Y., Jacks T. J. Spherosome membranes: half unit-membranes. Plant Physiol. 1972 Jun;49(6):937–943. doi: 10.1104/pp.49.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES