Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1986 Nov;82(3):695–699. doi: 10.1104/pp.82.3.695

Kinetic Properties of Phosphoenolpyruvate Carboxylase from C3, C4, and C3-C4 Intermediate Species of Flaveria (Asteraceae) 1

Hermann Bauwe 1,2, Raymond Chollet 1,2
PMCID: PMC1056191  PMID: 16665094

Abstract

Flaveria cronquistii (C3), F. chloraefolia (C3-C4), F. floridana (C3-C4), F. pubescens (C3-C4), F. anomala (C3-C4), F. linearis (C3-C4), F. brownii (C4), F. palmeri (C4), F. trinervia (C4) and F. australasica (C4), comprising 10 out of the 21 known species of the genus Flaveria (Asteraceae), were included in a comparative study of the kinetic and regulatory properties of green leaf phosphoenolpyruvate (PEP) carboxylase. At least three kinetically distinct enzyme-forms were identified on the basis of their affinities for PEP and the degree of allosterism with respect to this substrate. The kinetic properties of PEP carboxylase of most of the species seemingly were modified in vivo depending on the growth conditions of the plants. Km(PEPfree)-values of the enzyme from the five C3-C4 intermediate species ranged from 6 micromolar (F. chloraefolia, low light-grown) to 38 micromolar (F. pubescens, high light-grown). In contrast, the Km for PEP of PEP carboxylase from the C3 species F. cronquistii (13 micromolar) apparently was not influenced by growth conditions. The response of the enzyme from the C3 and C3-C4 species was hyperbolic in all cases. A second isoform with a lower affinity for PEP (88-100 micromolar), but also hyperbolic kinetics was found in the C4 species F. brownii, whereas in the three other C4 species examined a PEP carboxylase with a still lower affinity for PEP (187-221 micromolar) and sigmoidal kinetics was present. These isozyme-related kinetic data were supported by analyses of the elution behavior of the enzyme during anion-exchange chromatography on DEAE-Trisacryl M. The results are discussed with respect to the evolution of C4 photosynthesis in the Flaveria genus.

Full text

PDF
695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
  2. Huber S. C., Sugiyama T. Changes in Sensitivity to Effectors of Maize Leaf Phosphoenolypyruvate Carboxylase during Light/Dark Transitions. Plant Physiol. 1986 Jun;81(2):674–677. doi: 10.1104/pp.81.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Karabourniotis G., Manetas Y., Gavalas N. A. Detecting Photoactivation of Phosphoenolpyruvate Carboxylase in C(4) Plants : An Effect of pH. Plant Physiol. 1985 Feb;77(2):300–302. doi: 10.1104/pp.77.2.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ku M. S., Monson R. K., Littlejohn R. O., Nakamoto H., Fisher D. B., Edwards G. E. Photosynthetic Characteristics of C(3)-C(4) Intermediate Flaveria Species : I. Leaf Anatomy, Photosynthetic Responses to O(2) and CO(2), and Activities of Key Enzymes in the C(3) and C(4) Pathways. Plant Physiol. 1983 Apr;71(4):944–948. doi: 10.1104/pp.71.4.944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Manchester K. L. Determination of magnesium and potassium binding constants to phosphoenolpyruvate, 2- and 3-phosphoglycerate and a number of other anions. Biochim Biophys Acta. 1980 Jun 19;630(2):225–231. doi: 10.1016/0304-4165(80)90425-0. [DOI] [PubMed] [Google Scholar]
  6. Muss H. B., Bundy B. N., DiSaia P. J., Ehrlich C. E. Mitoxantrone for carcinoma of the endometrium: a phase II trial of the Gynecologic Oncology Group. Cancer Treat Rep. 1987 Feb;71(2):217–218. [PubMed] [Google Scholar]
  7. Rumpho M. E., Ku M. S., Cheng S. H., Edwards G. E. Photosynthetic Characteristics of C(3)-C(4) Intermediate Flaveria Species : III. Reduction of Photorespiration by a Limited C(4) Pathway of Photosynthesis in Flaveria ramosissima. Plant Physiol. 1984 Aug;75(4):993–996. doi: 10.1104/pp.75.4.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ting I. P., Osmond C. B. Photosynthetic phosphoenolpyruvate carboxylases: characteristics of alloenzymes from leaves of c(3) and c(1) plants. Plant Physiol. 1973 Mar;51(3):439–447. doi: 10.1104/pp.51.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES