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. 1986 Nov;82(3):771–779. doi: 10.1104/pp.82.3.771

A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium 1

Basic Parameters and Effects on Cell Growth

William B Terzaghi 1,2
PMCID: PMC1056206  PMID: 16665109

Abstract

The development of a system for modifying the membrane fatty acid composition of cultured soybean cells (Glycine max [L.] Merr.) is described. Tween-fatty acid esters carrying specific fatty acids were synthesized and added to the medium of suspension cultures. Cells transferred large quantities of exogenous fatty acids from Tweens to all acylated membrane lipids; up to 50% of membrane fatty acids were exogenously derived. C15 to C20 saturated fatty acids and C16, C18, and C20 unsaturated fatty acids with either cis or trans double bonds were incorporated into lipids. Cells elongated saturated fatty acids of C16 or less, and unsaturated fatty acids with cis double bonds were further desaturated. No other types of modifications were observed. Growth ceased in cells treated with excessive concentrations of Tween-fatty acid esters, but frequently not for several days. Cessation of cell growth was correlated with changes in membrane fatty acid composition resulting from incorporation of large amounts of exogenous fatty acids into membrane lipids, although cells tolerated large variations in fatty acid composition. Maximum tolerable Tween concentrations varied widely according to the fatty acid supplied. Potential uses of this system and implications of the observed modifications on the pathway of incorporation are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner R. R. Effect of unsaturated acids on membrane structure and enzyme kinetics. Prog Lipid Res. 1984;23(2):69–96. doi: 10.1016/0163-7827(84)90008-0. [DOI] [PubMed] [Google Scholar]
  2. Browse J., McCourt P., Somerville C. R. A mutant of Arabidopsis lacking a chloroplast-specific lipid. Science. 1985 Feb 15;227(4688):763–765. doi: 10.1126/science.227.4688.763. [DOI] [PubMed] [Google Scholar]
  3. Cassagne C., Lessire R. Biosynthesis of saturated very long chain fatty acids by purified membrane fractions from leek epidermal cells. Arch Biochem Biophys. 1978 Nov;191(1):146–152. doi: 10.1016/0003-9861(78)90076-0. [DOI] [PubMed] [Google Scholar]
  4. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  5. Hawke J. C., Stumpf P. K. The incorporation of oleic and linoleic acids and their desaturation products into the glycerolipids of maize leaves. Arch Biochem Biophys. 1980 Aug;203(1):296–306. doi: 10.1016/0003-9861(80)90180-0. [DOI] [PubMed] [Google Scholar]
  6. Lessire R., Stumpe P. K. Nature of the Fatty Acid Synthetase Systems in Parenchymal and Epidermal Cells of Allium porrum L. Leaves. Plant Physiol. 1983 Nov;73(3):614–618. doi: 10.1104/pp.73.3.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Omura S. Cerulenin. Methods Enzymol. 1981;72:520–532. [PubMed] [Google Scholar]
  8. Otoguro K., Awaya J., Tanaka H., Omura S. Saturated fatty acid-starved cells of Saccharomyces cerevisiae grown in the presence of cerulenin and oleic acid. J Biochem. 1981 Feb;89(2):523–529. doi: 10.1093/oxfordjournals.jbchem.a133228. [DOI] [PubMed] [Google Scholar]
  9. Radin N. S. Extraction of tissue lipids with a solvent of low toxicity. Methods Enzymol. 1981;72:5–7. doi: 10.1016/s0076-6879(81)72003-2. [DOI] [PubMed] [Google Scholar]
  10. Roughan P. G., Holland R., Slack C. R. The role of chloroplasts and microsomal fractions in polar-lipid synthesis from [1-14C]acetate by cell-free preparations from spinach (Spinacia oleracea) leaves. Biochem J. 1980 Apr 15;188(1):17–24. doi: 10.1042/bj1880017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Silbert D. F. Genetic modification of membrane lipid. Annu Rev Biochem. 1975;44:315–339. doi: 10.1146/annurev.bi.44.070175.001531. [DOI] [PubMed] [Google Scholar]
  12. Stumpf P. K., Weber N. Uptake and metabolism of fatty acids by soybean suspension cells. Lipids. 1977 Jan;12(1):120–124. doi: 10.1007/BF02532983. [DOI] [PubMed] [Google Scholar]
  13. Terzaghi W. B. Metabolism of tween-Fatty Acid esters by cultured soybean cells : kinetics of incorporation into lipids, subsequent turnover, and associated changes in endogenous Fatty Acid synthesis. Plant Physiol. 1986 Nov;82(3):780–786. doi: 10.1104/pp.82.3.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Waring A. J., Breidenbach R. W., Lyons J. M. In vivo modification of plant membrane phospholipid composition. Biochim Biophys Acta. 1976 Aug 16;443(2):157–168. doi: 10.1016/0005-2736(76)90499-5. [DOI] [PubMed] [Google Scholar]
  15. Wisnieski B. J., Williams R. E., Fox C. F. Manipulation of fatty acid composition in animal cells grown in culture. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3669–3673. doi: 10.1073/pnas.70.12.3669. [DOI] [PMC free article] [PubMed] [Google Scholar]

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