Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1986 Dec;82(4):1139–1144. doi: 10.1104/pp.82.4.1139

The Lipoxygenases in Developing Soybean Seeds, Their Characterization and Synthesis in Vitro1

Max O Funk 1,2,3, Richard T Carroll 1,2,3, John F Thompson 1,2,3, William R Dunham 1,2,3
PMCID: PMC1056272  PMID: 16665148

Abstract

A number of lipoxygenase isoenzymes were identified in developing soybean (Glycine max L. Merrill cv Provar) seeds and two have been partially characterized. In a study of lipoxygenase level in developing soybean seeds, the enzyme content increased markedly during development. Comparisons of the lipoxygenases from mature soybean seeds and immature seeds by isoelectric focusing, chromatofocusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping identified two categories of isoenzyme. The isoenzymes from immature seeds were found by electron paramagnetic resonance spectroscopy to be isolated at least in part as the high spin iron(III) or active form of the enzyme in contrast to lipoxygenases from mature seeds which were isolated as electron paramagnetic resonance silent, high spin iron(II) species. The discovery of increased levels of lipoxygenases during seed development and their isolation in an active form suggests that the enzyme may play a physiological role during the maturation process. The incorporation of iron-59 from the nutrient medium into lipoxygenase during culture of immature seeds was indicative of de novo synthesis of the enzyme. The efficiency of the iron uptake was high, as indicated by the level of radioactivity found in the enzyme (one gram atom of iron per mole of lipoxygenase).

Full text

PDF
1139

Images in this article

1141Fig. 1
on p.1141
1142Fig. 4
on p.1142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clapp C. H., Banerjee A., Rotenberg S. A. Inhibition of soybean lipoxygenase 1 by N-alkylhydroxylamines. Biochemistry. 1985 Apr 9;24(8):1826–1830. doi: 10.1021/bi00329a004. [DOI] [PubMed] [Google Scholar]
  2. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  3. Finazzi-Agrò A., Avigliano L., Veldink G. A., Vliegenthart J. F., Boldingh J. The influence of oxygen on the fluorescence of lipoxygenase. Biochim Biophys Acta. 1973 Dec 20;326(3):462–470. doi: 10.1016/0005-2760(73)90146-x. [DOI] [PubMed] [Google Scholar]
  4. Funk M. O., Whitney M. A., Hausknecht E. C., O'Brien E. M. Resolution of the isoenzymes of soybean lipoxygenase using isoelectric focusing and chromatofocusing. Anal Biochem. 1985 Apr;146(1):246–251. doi: 10.1016/0003-2697(85)90422-1. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K., Mölbert E., Showe M., Kellenberger E. Form-determining function of the genes required for the assembly of the head of bacteriophage T4. J Mol Biol. 1970 Apr 14;49(1):99–113. doi: 10.1016/0022-2836(70)90379-7. [DOI] [PubMed] [Google Scholar]
  6. Peterman T. K., Siedow J. N. Immunological comparison of lipoxygenase isozymes-1 and -2 with soybean seedling lipoxygenases. Arch Biochem Biophys. 1985 May 1;238(2):476–483. doi: 10.1016/0003-9861(85)90190-0. [DOI] [PubMed] [Google Scholar]
  7. Shingles R. M., Arron G. P., Hill R. D. Alternative pathway respiration and lipoxygenase activity in aged potato slice mitochondria. Plant Physiol. 1982 Jun;69(6):1435–1438. doi: 10.1104/pp.69.6.1435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Slappendel S., Veldink G. A., Vliegenthart J. F., Aasa R., Malmström B. G. EPR spectroscopy of soybean lipoxygenase-1. Determination of the zero-field splitting constants of high-spin Fe(III) signals from temperature and microwave frequency dependence. Biochim Biophys Acta. 1980 Jul 24;624(1):30–39. doi: 10.1016/0005-2795(80)90222-6. [DOI] [PubMed] [Google Scholar]
  9. Vernooy-Gerritsen M., Bos A. L., Veldink G. A., Vliegenthart J. F. Localization of lipoxygenases 1 and 2 in germinating soybean seeds by an indirect immunofluorescence technique. Plant Physiol. 1983 Oct;73(2):262–267. doi: 10.1104/pp.73.2.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vernooy-Gerritsen M., Leunissen J. L., Veldink G. A., Vliegenthart J. F. Intracellular localization of lipoxygenases-1 and -2 in germinating soybean seeds by indirect labeling with protein a-colloidal gold complexes. Plant Physiol. 1984 Dec;76(4):1070–1078. doi: 10.1104/pp.76.4.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vernooy-Gerritsen M., Veldink G. A., Vliegenthart J. F. Specificities of antisera directed against soybean lipoxygenases-1 and -2 and purification of lipoxygenase-2 by affinity chromatography. Biochim Biophys Acta. 1982 Nov 19;708(3):330–334. doi: 10.1016/0167-4838(82)90445-9. [DOI] [PubMed] [Google Scholar]
  12. Vick B. A., Zimmerman D. C. Biosynthesis of jasmonic Acid by several plant species. Plant Physiol. 1984 Jun;75(2):458–461. doi: 10.1104/pp.75.2.458. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES