Abstract
A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H2O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.
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Selected References
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- Flores H. E., Galston A. W. Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol. 1982 Mar;69(3):701–706. doi: 10.1104/pp.69.3.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seiler N., Knödgen B. Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethylaminonaphthalene-1-sulfonyl derivatives. J Chromatogr. 1978 Jan 1;145(1):29–39. doi: 10.1016/s0378-4347(00)81665-5. [DOI] [PubMed] [Google Scholar]
- Seiler N., Wiechmann M. Die Mikrobestimmung von Spermin und Spermidin als 1-Dimethylamino-naphthalin-5-sulfonsäure-Derivate. Hoppe Seylers Z Physiol Chem. 1967 Oct;348(10):1285–1290. [PubMed] [Google Scholar]
- Tabor C. W., Tabor H. Polyamines. Annu Rev Biochem. 1984;53:749–790. doi: 10.1146/annurev.bi.53.070184.003533. [DOI] [PubMed] [Google Scholar]