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. 1987 Feb;83(2):259–261. doi: 10.1104/pp.83.2.259

Glutamine Synthetase Isoenzymes in the Green Soil Alga Stichococcus bacillaris Naeg. 1

Iftikhar Ahmad 1, Johan A Hellebust 1
PMCID: PMC1056344  PMID: 16665232

Abstract

Two forms of glutamine synthetase (GS1 and GS2) have been separated from cells of Stichococcus bacillaris by fast protein liquid chromatography. The activities of the two isoenzymes were influenced by the composition of the media employed; thiol reagents were essential for stabilizing GS2 but they suppressed GS1 activity. The activity of each isoenzyme was, therefore, determined following separate purification procedures. Growth conditions influenced both isoenzymes; GS2 showed maximum activity under photoautotrophic conditions, whereas GS1 showed maximum activity under heterotrophic conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmad I., Hellebust J. A. Nitrogen Metabolism of the Marine Microalga Chlorella autotrophica. Plant Physiol. 1984 Nov;76(3):658–663. doi: 10.1104/pp.76.3.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hirel B., Gadal P. Glutamine Synthetase in Rice: A COMPARATIVE STUDY OF THE ENZYMES FROM ROOTS AND LEAVES. Plant Physiol. 1980 Oct;66(4):619–623. doi: 10.1104/pp.66.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Mann A. F., Fentem P. A., Stewart G. R. Identification of two forms of glutamine synthetase in barley (Hordeum vulgare). Biochem Biophys Res Commun. 1979 May 28;88(2):515–521. doi: 10.1016/0006-291x(79)92078-3. [DOI] [PubMed] [Google Scholar]
  4. McNally S. F., Hirel B., Gadal P., Mann A. F., Stewart G. R. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf. Plant Physiol. 1983 May;72(1):22–25. doi: 10.1104/pp.72.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Shelp B. J., Canvin D. T. Photorespiration and Oxygen Inhibition of Photosynthesis in Chlorella pyrenoidosa. Plant Physiol. 1980 May;65(5):780–784. doi: 10.1104/pp.65.5.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sumar N., Casselton P. J., McNally S. F., Stewart G. R. Occurrence of Isóenzymes of Glutamine Synthetase in the Alga Chlorella kessleri. Plant Physiol. 1984 Feb;74(2):204–207. doi: 10.1104/pp.74.2.204. [DOI] [PMC free article] [PubMed] [Google Scholar]

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