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. 1987 Feb;83(2):395–398. doi: 10.1104/pp.83.2.395

myo-Inositol Trisphosphate Mobilizes Calcium from Fusogenic Carrot (Daucus carota L.) Protoplasts 1

Magaly Rincon 1, Wendy F Boss 1
PMCID: PMC1056368  PMID: 16665256

Abstract

To determine whether or not inositol trisphosphate (IP3) mobilizes calcium in higher plant cells, we investigated the effect of IP3 on Ca2+ fluxes in fusogenic carrot (Daucus carota L.) protoplasts. The protoplasts were incubated in 45Ca2+-containing medium and the 45Ca2+ associated with the protoplasts was monitored with time. Addition of IP3 (20 micromolar) caused a 17% net loss of the accumulated 45Ca2+ within 4 minutes. There was a reuptake of 45Ca2+ and the protoplasts recovered to their initial value by 10 minutes. Phytic acid (IP6), also stimulated 45Ca2+ efflux from the protoplasts. Both the IP3− and the IP6−induced 45Ca2+ efflux were inhibited by the calmodulin antagonist, trifluoperazine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. J., Heslop J. P., Irvine R. F., Brown K. D. Inositol trisphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor. Biochem J. 1984 Aug 15;222(1):195–201. doi: 10.1042/bj2220195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. J. Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol. Biochem J. 1983 Jun 15;212(3):849–858. doi: 10.1042/bj2120849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boss W. F., Massel M. O. Polyphosphoinositides are present in plant tissue culture cells. Biochem Biophys Res Commun. 1985 Nov 15;132(3):1018–1023. doi: 10.1016/0006-291x(85)91908-4. [DOI] [PubMed] [Google Scholar]
  4. Creba J. A., Downes C. P., Hawkins P. T., Brewster G., Michell R. H., Kirk C. J. Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormones. Biochem J. 1983 Jun 15;212(3):733–747. doi: 10.1042/bj2120733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Drøbak B. K., Ferguson I. B. Release of Ca2+ from plant hypocotyl microsomes by inositol-1,4,5-trisphosphate. Biochem Biophys Res Commun. 1985 Aug 15;130(3):1241–1246. doi: 10.1016/0006-291x(85)91747-4. [DOI] [PubMed] [Google Scholar]
  6. Grimes H. D., Boss W. F. Intracellular calcium and calmodulin involvement in protoplast fusion. Plant Physiol. 1985 Sep;79(1):253–258. doi: 10.1104/pp.79.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirata M., Suematsu E., Hashimoto T., Hamachi T., Koga T. Release of Ca2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphate. Biochem J. 1984 Oct 1;223(1):229–236. doi: 10.1042/bj2230229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Michell B. Inositol phosphates. Profusion and confusion. Nature. 1986 Jan 16;319(6050):176–177. doi: 10.1038/319176a0. [DOI] [PubMed] [Google Scholar]
  10. Nishizuka Y. Turnover of inositol phospholipids and signal transduction. Science. 1984 Sep 21;225(4668):1365–1370. doi: 10.1126/science.6147898. [DOI] [PubMed] [Google Scholar]
  11. Streb H., Irvine R. F., Berridge M. J., Schulz I. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate. Nature. 1983 Nov 3;306(5938):67–69. doi: 10.1038/306067a0. [DOI] [PubMed] [Google Scholar]
  12. Trewavas A. J., Sexton R., Kelly P. Polarity, calcium and abscission: molecular bases for developmental plasticity in plants. J Embryol Exp Morphol. 1984 Nov;83 (Suppl):179–195. [PubMed] [Google Scholar]

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