Abstract
The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Foyer C., Walker D., Spencer C., Mann B. Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem J. 1982 Feb 15;202(2):429–434. doi: 10.1042/bj2020429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerhardt R., Heldt H. W. Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media. Plant Physiol. 1984 Jul;75(3):542–547. doi: 10.1104/pp.75.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giersch C., Heber U., Kaiser G., Walker D. A., Robinson S. P. Intracellular metabolite gradients and flow of carbon during photosynthesis of leaf protoplasts. Arch Biochem Biophys. 1980 Nov;205(1):246–259. doi: 10.1016/0003-9861(80)90105-8. [DOI] [PubMed] [Google Scholar]
- Hampp R., Goller M., Füllgraf H. Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymic cycling. Plant Physiol. 1984 Aug;75(4):1017–1021. doi: 10.1104/pp.75.4.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heber U., Pon N. G., Heber M. Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts. Plant Physiol. 1963 May;38(3):355–360. doi: 10.1104/pp.38.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heldt W. H., Werdan K., Milovancev M., Geller G. Alkalization of the chloroplast stroma caused by light-dependent proton flux into the thylakoid space. Biochim Biophys Acta. 1973 Aug 31;314(2):224–241. doi: 10.1016/0005-2728(73)90137-0. [DOI] [PubMed] [Google Scholar]
- Herzog B., Stitt M., Heldt H. W. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : III. Properties of the Cytosolic Fructose 1,6-Bisphosphatase. Plant Physiol. 1984 Jul;75(3):561–565. doi: 10.1104/pp.75.3.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huber S. C., Bickett D. M. Evidence for control of carbon partitioning by fructose 2,6-bisphosphate in spinach leaves. Plant Physiol. 1984 Feb;74(2):445–447. doi: 10.1104/pp.74.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lilley R. M., Chon C. J., Mosbach A., Heldt H. W. The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim Biophys Acta. 1977 May 11;460(2):259–272. doi: 10.1016/0005-2728(77)90212-2. [DOI] [PubMed] [Google Scholar]
- Rufty T. W., Huber S. C. Changes in Starch Formation and Activities of Sucrose Phosphate Synthase and Cytoplasmic Fructose-1,6-bisphosphatase in Response to Source-Sink Alterations. Plant Physiol. 1983 Jun;72(2):474–480. doi: 10.1104/pp.72.2.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rufty T. W., Kerr P. S., Huber S. C. Characterization of diurnal changes in activities of enzymes involved in sucrose biosynthesis. Plant Physiol. 1983 Oct;73(2):428–433. doi: 10.1104/pp.73.2.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sicher R. C., Kremer D. F. Changes of Sucrose-Phosphate Synthase Activity in Barley Primary Leaves during Light/Dark Transitions. Plant Physiol. 1984 Dec;76(4):910–912. doi: 10.1104/pp.76.4.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stitt M., Cseke C., Buchanan B. B. Regulation of fructose 2,6-bisphosphate concentration in spinach leaves. Eur J Biochem. 1984 Aug 15;143(1):89–93. doi: 10.1111/j.1432-1033.1984.tb08345.x. [DOI] [PubMed] [Google Scholar]
- Stitt M., Gerhardt R., Kürzel B., Heldt H. W. A role for fructose 2,6-bisphosphate in the regulation of sucrose synthesis in spinach leaves. Plant Physiol. 1983 Aug;72(4):1139–1141. doi: 10.1104/pp.72.4.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stitt M., Herzog B., Heldt H. W. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : I. Coordination of CO(2) Fixation and Sucrose Synthesis. Plant Physiol. 1984 Jul;75(3):548–553. doi: 10.1104/pp.75.3.548. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stitt M., Herzog B., Heldt H. W. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : V. Modulation of the Spinach Leaf Cytosolic Fructose 1,6-Bisphosphatase Activity in Vitro by Substrate, Products, pH, Magnesium, Fructose 2,6-Bisphosphate, Adenosine Monophosphate, and Dihydroxyacetone Phosphate. Plant Physiol. 1985 Nov;79(3):590–598. doi: 10.1104/pp.79.3.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stitt M., Kürzel B., Heldt H. W. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : II. Partitioning between Sucrose and Starch. Plant Physiol. 1984 Jul;75(3):554–560. doi: 10.1104/pp.75.3.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stitt M., Wirtz W., Heldt H. W. Metabolite levels during induction in the chloroplast and extrachloroplast compartments of spinach protoplasts. Biochim Biophys Acta. 1980 Nov 5;593(1):85–102. doi: 10.1016/0005-2728(80)90010-9. [DOI] [PubMed] [Google Scholar]
- Stitt M., Wirtz W., Heldt H. W. Regulation of Sucrose Synthesis by Cytoplasmic Fructosebisphosphatase and Sucrose Phosphate Synthase during Photosynthesis in Varying Light and Carbon Dioxide. Plant Physiol. 1983 Jul;72(3):767–774. doi: 10.1104/pp.72.3.767. [DOI] [PMC free article] [PubMed] [Google Scholar]