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. 2023 Jul 30;11(10):6393–6402. doi: 10.1002/fsn3.3577

Anticancer assessment and antibiofilm potential of Laetiporus sulphureus mushroom originated from Serbia

Milena M Jovanović 1, Katarina G Marković 2, Mirjana Ž Grujović 2, Jelena Pavić 2, Milan Mitić 3, Jelena Nikolić 3, Dragana Šeklić 2,✉
PMCID: PMC10563721  PMID: 37823160

Abstract

Laetiporus sulphureus (Bull.) Murrill is a well‐known edible mushroom consumed in nutrition as delicacy. It has been used in traditional medicine because of its beneficial effects on human wellness, such as antimicrobial, antioxidant, and anticancer potential. The present study determined the phenolic profile of Laetiporus sulphureus ethanolic extract (LSE) by high‐performance liquid chromatographic method. Tolerance of two probiotic bacterial strains Lactiplantibacillus plantarum 229v, Bifidobacterium animalis subsp. lactis and probiotic yeast Saccharomyces boulardii on LSE was analyzed in terms of viability and biofilm formation. Effects of extract on colorectal (HCT‐116) and cervical (HeLa) cancer cells viability was determined using MTT test in concentration range: 1–500 μg/mL after 24 and 72 h. Redox parameters (superoxide anion radicals, nitrites, and reduced glutathione) were evaluated using NBT, Griess, and GSH assays in the concentration range of 1–500 μg/mL after 24 and 72 h. Antimigratory activity was determined by wound healing method using selected concentrations of 10 and 50 μg/mL after 24 h. Untreated cells were considered as control. As control cell line, we used healthy fibroblasts (MRC‐5). Our results demonstrated abundance of LSE in phenolics, with rosmarinic acid as the main component. LSE induced low tolerance of tested planktonic probiotic strains, with no affection on their ability to form biofilm. No significant cytotoxicity on tested cancer cells was observed, with prooxidative and antimigratory effects noticed. Extract exerted significant antimigratory activity on cancer cells without effect on planktonic and probiotic cultures in biofilm. These results indicate potential application of Laetiporus sulphureus ethanolic extract as natural protector of probiotics with prominent ability to suppress cancer cell motility.

Keywords: antibiofilm activity, migration, mushroom, phenolic profile, probiotics

Rosmarinic acid is the main component in Laetiporus sulphureus ethanolic extract. Prooxidative and antimigratory effects were observed on tested cancer cells. Low doses of our extract showed promising results on tested model systems regarding significant antimigratory capacity for cancer cells without effect on planktonic and probiotic cultures in biofilm.

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1. INTRODUCTION

Cancer is a significant global health problem and a pertinent cause of death (Lee et al., 2019). Colorectal (CRC) and cervical cancer are among the most common types of cancers with high incidence and mortality rates (Cohen et al., 2019). For cancer progression, in terms of metastasis and resistance to therapeutic standards, redox status and migratory potential of cancer cells play a very important role (Xing et al., 2022). It is known that redox disbalance in cancer cells influences the regulation of cell movement (Xing et al., 2022); therefore, it is important to examine the connection between these two mechanisms. Also, many studies confirmed that the diversity of microflora plays an important role in occurrence and development of this disease (Bhatt et al., 2017; Cohen et al., 2019).

Probiotics are defined as nonpathogenic microorganisms with positive impact on medical condition of their host (FAO/WHO, 2006; Kiani, Nami, Hedayati, Elieh Ali Komi, et al., 2021; Kiani, Nami, Hedayati, Jaymand, et al., 2021). Probiotic strains are mostly lactic acid bacteria, and species that belong to genera Lactobacillus, Bifidobacterium, Streptococcus, Lactococcus, and Enterococcus are the most commonly used as probiotic cultures (Kiani, Nami, Hedayati, Elieh Ali Komi, et al., 2021; Kiani, Nami, Hedayati, Jaymand, et al., 2021). Traditionally used dairy products are regarded as the main source of these probiotic strains (Kiani, Nami, Hedayati, Elieh Ali Komi, et al., 2021; Kiani, Nami, Hedayati, Jaymand, et al., 2021), while they can also be found in fermented and nonfermented food products, such as cereals, fruits, vegetables, meats, and fish (Damián et al., 2022; Zommiti et al., 2020). Gastrointestinal flora consists of approximately 300–500 bacterial species, whereat Bacteroides, Porphyromonas, Bifidobacterium, Lactobacillus, Enterococcus, and Clostridium have been recognized as the most dominant in human gastrointestinal tract (Quigley, 2013). The importance and application of probiotic strains are multiple considering that they possess proven therapeutic effects (Kiani, Nami, Hedayati, Elieh Ali Komi, et al., 2021; Kiani, Nami, Hedayati, Jaymand, et al., 2021). Some of them are: regulation of gut microbiota population, proper epithelial function, improving immune response, inhibition of intestinal infections, improvement of digestion, lowering blood cholesterol, reducing the irritable bowel syndrome and inflammatory bowel disease, suppression of diarrhea, rheumatoid arthritis, atopic dermatitis, antihypertensive, antiallergic, reduction of active ulcerative colitis, anticancer activity, and many more (Kahieshesfandiari et al., 2021; Kiani, Nami, Hedayati, Elieh Ali Komi, et al., 2021; Kiani, Nami, Hedayati, Jaymand, et al., 2021; Sánchez et al., 2017; Zommiti et al., 2020). Their use is beneficial in the regulation of damaged microflora in various cancers, as a consequence of the application of anticancer treatments with harmful side effects (Lu et al., 2021).

Proper nutritive habits are required for the function of healthy human organism, and their importance have been recognized for cancer patients (Key et al., 2020). Natural compounds are potential adjuvants to cancer therapy, and mushrooms with their proven nutritive properties are consumed by humans because of their multiple biological activities (Ma et al., 2018).

Cancer fungotherapy presents a promising scientific field and is focused on recognition of substances derived from mushrooms that exert antitumor activity (Blagodatski et al., 2018). In many studies, researchers investigated the cytotoxic effects of various mushroom extracts on cancer cells (Blagodatski et al., 2018; Klaus et al., 2013; Šeklić et al., 2016).

Laetiporus sulphureus (Bull.) Murrill is an edible mushroom species that possess proven antimicrobial, antioxidative, and anticancer activities (Khalilov et al., 2019; Younis et al., 2019). L. sulphureus is a valuable source of antioxidants (Klaus et al., 2013); however, data regarding its effects on redox status in relation to migratory potential of cancer cells are scarce (Klaus et al., 2013; Petrović, Papandreou, et al., 2014; Petrović, Stojković, et al., 2014). Numerous studies were focused on the effects of L. sulphureus on enterobacteria and antibacterial activity; yet, there is a lack of information regarding their influence on the probiotic bacteria.

This study aimed to provide an overview of the most important phenolic components and biological activity of the L. sulphureus ethanolic extract (LSE). The main focus is examination of LSE effects on probiotic strains, and the preservation of their biofilm. Also, anticancer effects in terms of cytotoxic, antioxidant, and antimigratory potential were analyzed on human healthy cell line MRC‐5, colorectal cancer cell line HCT‐116, and cervical adenocarcinoma cell line HeLa.

2. MATERIALS AND METHODS

2.1. Mushroom samples and extractions

Laetiporus sulphureus was gathered from lying tree trunks of Salix sp. in Šumadija area, Serbia (43°54′00.32″ N, 20°52′02.90″ E, altitude 629 m) during the autumn of 2019. Identification and classification of the mushroom were performed by standard keys by Mycological society “Šumadija” (Kragujevac, Serbia). Collection of dried and material extracted by ethanol from mushroom thalli were previously described by Šeklić et al. (2016). The obtained amount of crude L. sulphureus ethanolic extract was 1.92 g.

2.2. High‐performance liquid chromatographic (HPLC) profile

Quantification of individual phenolic compounds was performed using reversed‐phase HPLC analysis. The equipment used was an HPLC Agilent‐1200 series with Ultraviolet–Visible DAD detector for multiwavelength detection. After injecting 5 μL of sample (100 mg of crude extract in 1 mL 100% DMSO), the separation was performed in an Agilent‐Eclipse XDB C‐18 column (4.6 × 150 mm), which was thermostated at 25°C. Two solvents were used for the gradient elution: A (H2O + 5%HCOOH) and B (80%ACN + 5%HCOOH + H2O). The elution program used was as follows: from 0 to 10 min 0% B, from 10 to 28 min gradually increased 0%–25% B, from 28 to 30 min 25% B, from 30 to 35 min gradually increased 25–50% B, from 35 to 40 min gradually increased 50%–80% B, and finally for the last 5 min gradually decreased 80%–0% B. Phenolic compounds in the samples were identified by comparing their retention times and spectra with retention time and spectra of standards for each component. Quantitative data were calculated from the calibration curves. For each standard, stock solutions were prepared in 10% (v/v) methanol. Concentrations of components in the samples were calculated from the equation obtained from calibration graphs, constructed for each standard. All chemicals and solvents were of analytical or HPLC purity. All analyses were performed in triplicate and results were expressed as milligrams per 1 g of dry extract weight.

2.3. Tolerance assessment of probiotics in the presence of mushroom extract

The tolerance of selected probiotic microorganisms to the presence of L. sulphureus extract was tested on two bacterial probiotic strains: Lactiplantibacillus plantarum 229v and Bifidobacterium animalis subsp. lactis and one strain of probiotic yeast: Saccharomyces boulardii. We chose these probiotic strains since they are the most present in commercial probiotics products. Further, their preventive and therapeutic effect on human health is well known (Grujović et al., 2022). All strains were provided by the Faculty of Science, University of Kragujevac, Serbia. Bacterial and yeast suspensions were prepared by the direct colony method (Andrews, 2005). The activity of LSE on probiotics was tested by determining the minimum inhibitory concentration (MIC) and minimum microbiocidal concentration (MMC) (Sarker et al., 2007). Tetracycline (Sigma Chemicals Co.) and fluconazole (Pfizer Inc.), dissolved in the nutrient liquid medium, were used as reference compounds. Stock solutions of crude extracts were obtained by dissolving them in 10% DMSO, which was used as a control. Each test included growth control and sterility control. All tests were performed in duplicates and mean values were presented.

2.4. The ability of tested probiotics to form a biofilm

The ability of L. plantarum 229v, B. animalis subsp lactis, and S. boulardii to form biofilms was assayed as described by O'Toole and Kolter (1998) with some modifications. The tissue culture 96‐well microtiter plates (Sarstedt) were prepared by dispensing 50 mL of MRS broth (Torlak) for bacteria or SAB broth (Torlak) for yeast into each well. Fresh bacterial or yeast suspension was added to each well. The inoculated microtiter plates were incubated at 37°C for 24 h for bacteria and at 28°C for 48 h for yeast. After incubation, the content of each well was gently removed by tapping the microtiter plates. The wells were washed with 200 mL of sterile 0.85% saline to remove free‐floating bacteria. Biofilms formed by adherent cells in 96‐well microtiter plates were stained with crystal violet (0.1% w/v; Fluka AG) and incubated at room temperature for 20 min. The excess stain was rinsed off by thorough washing with deionized water and then with 200 mL of 96% ethanol. Optical densities (ODs) of stained adherent bacteria were determined with an enzyme‐linked immunosorbent assay (ELISA) plate reader (RT‐2100C; Rayto) at 630 nm wavelength. The experiment included the positive control, the Staphylococcus epidermidis ATCC 35984, a strong biofilm‐producing strain, and the negative control containing only the culture medium (MRS or SAB). The biofilm formation assay was performed in four replicate wells for each strain and results were presented as means ± standard deviations.

According to Stepanović et al. (2000), the cut‐off O.D. (O.Dc) was defined as three standard deviations above the mean O.D. of the negative controls. Strains were classified as follows: O.D. < O.Dc—no biofilm producer, O.Dc < O.D. < 2O.Dc—weak biofilm producer, 2O.Dc < O.D. < 4O.Dc—moderate biofilm producer and 4O.Dc < O.D.—strong biofilm producer.

2.4.1. Tolerance assessment of probiotic biofilm formation in the presence of mushroom extract

The tissue culture 96‐well microtiter plates were prepared by dispensing 50 μL of broth into each well. Two‐fold serial dilutions of tested extracts were made using a multichannel pipette following which 50 mL of fresh bacterial suspension was added to each well. The inoculated microtiter plates were incubated at 37°C for 24 h for bacteria and at 28°C for 48 h for yeast. The rest of the experiment was done as described by Muruzović et al. (2016). The biofilm inhibitory concentration required to reduce biofilm coverage by 50% (BIC50) or 90% (BIC90) was defined as the lowest concentration of extract that showed 50% or 90% inhibition on the biofilm formation (Chaieb et al., 2011).

Only broth or broth with extracts served as a control to check sterility and nonspecific binding of media. To compensate for background absorbance, OD readings from sterile medium, extracts, fixative, and dye were averaged and subtracted from all test values. All tests were performed in duplicate. Tetracycline and fluconazole, dissolved in the liquid medium, were used as reference compounds.

2.5. Determination of anticancer activity

Colorectal carcinoma and cervical adenocarcinoma cancer cell lines (HCT‐116, HeLa), as well as healthy fibroblasts (MRC‐5), were seeded and treated for MTT, NBT, Griess assays, and glutathione (GSH) tests using standard procedures (Šeklić et al., 2016). Cells were treated with LSE working concentrations 1, 10, 50, 100, 250, and 500 μg/mL, while untreated cells served as a negative control. The results were evaluated 24 and 72 h after treatment, and absorbances were measured on ELISA reader (Elisa Microplate Reader RT‐6100; Rayto). Wound healing assay was used for the determination of antimigratory potential of LSE (concentrations 10 and 50 μg/mL), and results were determined after 24 h. Effects were analyzed on HCT‐116 and HeLa cells, and assay protocol has been already described by Kosanić et al. (2020).

2.6. Statistical analysis

Statistical analyses were performed using statistical software package (SPSS for Windows, ver. 17, 2008), whereat data were assessed by one‐way analysis of variance (ANOVA). The IC50 values (concentration of the effect which inhibits 50% cell growth) were calculated from calibration curve by CalcuSyn program (CalcuSyn, v. 2.1; BioSoft; 2009).

3. RESULTS

3.1. Chemical profile of L. sulphureus extract

The contents of individual phenolic compounds from analyzed extract are presented in Table 1.

TABLE 1.

Phenolic compounds content in Laetiporus sulphureus ethanolic extract (mg/g DW).

Compound Ethanol extract
Vanillic acid 0.376 ± 0.002
Epicatechin 0.236 ± 0.001
Naringenin 0.0819 ± 0.0003
Ferulic acid 0.03250 ± 0.0006
Rosmarinic acid 0.416 ± 0.003
p‐hydroxybenzoic acid 0.342 ± 0.002
Luteolin 0.0247 ± 0.000
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Note: Results are presented as average value of three repeats ± SD.

HPLC chromatograms of analyzed extract are presented in Figure 1.

FIGURE 1.

FIGURE 1

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High‐performance liquid chromatographic chromatogram of Laetiporus sulphureus ethanolic extract at 280 nm (a) and 320 nm (b).

The following compounds were identified in LSE: vanillic acid, epicatechin, naringenin, ferulic acid, rosmarinic acid, p‐hydroxybenzoic acid, and luteolin. The main component detected in extract was rosmarinic acid (0.416 ± 0.003 mg/1 g DW). This phenolic was found in many plants, and it has proven antioxidant, anti‐inflammatory, antimutagenic, antitumor, antigenotoxic, cytotoxic, antimetastatic, antiangiogenic, neuroprotective, antimicrobial, immunomodulatory, melanogenic, and antivenom effects (Amoah et al., 2016). Vanillic acid and p‐hydroxybenzoic acid were also found in high amount (0.376 and 0.342 mg/1 g DW, respectively).

3.2. Tolerance assessment of probiotics in the presence of mushroom extract

The results of in vitro tolerance of tested probiotics in the presence of L. sulphureus ethanol extract, determined by MICs and MMCs, are shown in Table 2. The results were compared with the influence of tetracycline for bacteria and fluconazole for yeast.

TABLE 2.

Determination of Laetiporus sulphureus ethanolic extract (LSE) antimicrobial activity.

Species of bacteria/yeast LSE Tetracycline Fluconazole
MIC MMC MIC MMC MIC MMC
L. plantarum 229v 0.031 0.062 0.16 1 / /
B. animalis subsp lactis 0.058 0.078 4 8 / /
S. boulardii 0.14 0.28 / / 7.81 31.25
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Note: Minimum inhibitory (MIC) and minimum microbiocidal concentration (MMC) are given in mg/mL for extract and μg/mL for tetracycline and fluconazole.

The bacterial probiotic strains were sensitive to the tested extract (MIC and MMC values from 0.031 to 0.078 mg/mL), while S. boulardii showed a lower sensitivity compared to bacteria (MIC and MMC values ranged from 0.14 to 0.28 mg/mL). According to Muruzović et al. (2016), 10% DMSO had no inhibitory potential on the growth of microorganisms.

3.3. Tolerance assessment of probiotic biofilm formation in the presence of mushroom extract

The ability of tested probiotic species to form biofilms was quantified by measuring the absorbance of stained biofilms at 630 nm with an ELISA plate reader. The results indicated that all tested bacteria showed the ability to form biofilms. The absorbance values were 0.13 for L. plantarum 229v, 0.10 for B. animalis subsp lactis, and 0.08 for S. boulardii. According to Stepanović et al. (2000), the tested probiotic strains were weak biofilm producers. The results of probiotic biofilm formation tolerance in the presence of the LSE are presented in Table 3.

TABLE 3.

Antibiofilm activity of Laetiporus sulphureus ethanolic extract (LSE).

Species of bacteria/yeast LSE Tetracycline Fluconazole
BIC50 BIC90 BIC50 BIC90 BIC50 BIC90
L. plantarum 229v 8.76 >10 9.30 31.25 / /
B. animalis subsp lactis 7 10 28.9 62.5 / /
S. boulardii 1.13 5 / / 790 >1000
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Note: Values are given in mg/mL for extract and μg/mL for tetracycline and fluconazole.

BIC50 was defined as the lowest concentration of extract that showed 50% inhibition on the biofilm formation, while BIC90 was defined as the lowest concentration of extract that showed 90% inhibition on the biofilm formation. The lowest tolerance to the tested extract was detected in the biofilm formation of S. boulardii (BIC50 at 1.13 mg/mL, while BIC90 for this extract was at 5 mg/mL) (Table 3).

3.4. Determination of anticancer activity

3.4.1. Cytotoxic effects

The cytotoxic effects of L. sulphureus ethanol extract on tested cell lines were investigated by MTT assay. According to the cytotoxicity criteria of whole crude extracts (Itharat et al., 2004), the IC50 values below 30 μg/mL indicate that extract could be considered as effective cytostatic. The results of the present study showed no cytotoxic effects of LSE (IC50 > 300 μg/mL) on all tested cell lines, whereat the weakest effect was detected on healthy cell line, while the strongest cytotoxic activity was observed on HeLa cells.

3.4.2. Effects on redox status

We employed NBT assay for quantification of superoxide anion radicals' level in control and treated MRC‐5, HCT‐116, and HeLa cells after 24 and 72 h of incubation (results are depicted in Figure 2). Measurement on MRC‐5 cells revealed antioxidative effects of treatment after 72 h. Reduction of O₂˙ˉ and nitrite level was followed by the same trend regarding GSH concentration.

FIGURE 2.

FIGURE 2

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Effects of Laetiporus sulphureus ethanolic extract on redox status parameters in MRC‐5 (a), HCT‐116 (b), and Hela (c) cells. Results are presented as means ± SE of three independent experiments performed in three repeats.

In HCT‐116 cells, basal expression of O₂˙ˉ and NO₂ˉ was observed in higher levels when compared to healthy cells (Figure 2b). This cell line was sensitive to LSE extract which exerted significant acute prooxidative effect, elevating both O₂˙ˉ and NO₂ˉ. Basal level of GSH in HCT‐116 cells was observed lower comparing to MRC‐5 and HeLa cells, which indicate weaker antioxidative protection.

When it comes to HeLa cancer cells, basal level of O₂˙ˉ is higher than in other tested cell lines (Figure 2c). HeLa cells were the most sensitive to treatment in terms of O₂˙ˉ production. LSE triggered acute superoxide anion radical production (Figure 2c). Regarding levels of nitrites and GSH, elevation was observed only in higher treatment concentrations.

In general, LSE induced antioxidative effects on healthy cells, while prooxidative on cancer cell lines, especially on HCT‐116 colorectal cancer cells.

3.4.3. Antimigratory activity

In the present examination, effects of extract were evaluated for its antimigratory activity in two selected concentrations, 10 and 50 μg/mL. LSE showed no effect on migration of healthy MRC‐5 cells (Figure 3a), while HCT‐116 and HeLa responded as sensitive cell lines to this treatment. LSE inhibited significantly HCT‐116 and HeLa cells migration, whereat HCT‐116 appeared to be the most sensitive to the treatment. Higher applied concentration of this extract exerted the strongest antimigratory effects on colorectal carcinoma cells (Figure 3b,c).

FIGURE 3.

FIGURE 3

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Effects of LSE on migration of MRC‐5 (a), HCT‐116 (b), and HeLa (c) cell lines. Analysis of wound space is shown as relative level of changes in wound space width. Results are presented as means ± SE of three independent experiments performed in three repeats. *p < .05 is considered as statistically significant difference between treatments and control compared to 0 h.

4. DISCUSSION

Ethanol, as the most efficient solvent in terms of extraction of phenolic compounds from mushrooms, was used in preparation of many plant tinctures with application in traditional medicine (Sezgin et al., 2019). Also, the highest yield of polyphenolic compounds with known antioxidant activity is extracted with ethanolic solvent (Akinmoladun et al., 2010; Elekofehinti & Kade, 2012; Gryglewski et al., 1987). Those compounds, naturally present in plants and mushrooms, have potential to protect from various diseases, including cancer. The main component of analyzed LSE was rosmarinic acid with known effectiveness against cancer and it could be used as therapeutic agent (Hossan et al., 2014). Its mechanism of action is primarily anti‐inflammatory and free radical scavenging, which inhibits cell proliferation, migration, and selective induction of cancer cell apoptosis (Hossan et al., 2014). As our results present, vanillic acid component is also found in high amount in this extract. According to the literature data, no cytotoxic, mutagenic, and antimutagenic effects of different concentrations of vanillic acid were demonstrated so far (Almeida et al., 2016).

Many studies from various countries analyzed ethanol extract of L. sulphureus using HPLC method, and many phenolics have been detected, such as quercetin, kaempferol, myricetin, luteolin, catechin, gallic, and phenolic acids (chlorogenic, caffeic, p‐coumaric, cinnamic, p‐hydroxybenzoic, 4‐hydroxybenzoic, salicylic, trans‐cinnamic, vanillic) (Olennikov et al., 2011; Petrović, Papandreou, et al., 2014; Petrović, Stojković, et al., 2014), which is in concordance with the present study.

L. sulphureus has good antibiotic activity against Candida albicans ATCC 10321, Candida parapsilosis CBS604, Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538, Enterococcus faecalis, and Staphylococcus epidermidis ATCC12228c as shown in Petrović et al. (2013) and Popa et al. (2016). In this paper, for the first time, the tolerance of probiotic species to the LSE was investigated and the results indicated their high sensitivity.

We investigated the influence of LSE on the inhibition of biofilm formation by some probiotic strains. The tested planktonic probiotic strains showed low tolerance to this treatment, while their ability to form biofilm was not affected.

Examination of migratory potential presents the basis of cancer research, and suppression of migration is essential for the treatment of cancer. Therefore, studies regarding antimigratory activity of natural products are relatively scarce. It is known that cancer cell motility is under significant regulation by redox status (Xing et al., 2022).

Our research shows that ethanol extract and detected phenolic compounds within possess an antioxidant effect in healthy cells, indicating its protective potential on healthy cells from free radicals (Elekofehinti & Kade, 2012). However, in cells with disturbed redox balance, such as cancer cells, polyphenolic compounds can have a prooxidative effect (Liou & Storz, 2010; Šeklić et al., 2018). Based on the results of this study, we can conclude that the tested LSE extract exerted better effects on HCT‐116 cells. These cells are proved to be very sensitive to natural treatments which are able to induce prooxidative effects (Šeklić et al., 2018). This can be explained by the molecular profile of HCT‐116 cells, with defective reparation gene MLH‐1 (Hassen et al., 2016). MLH1‐deficient cells have a significant reduction in activity of the respiratory chain Complex I and functional consequence of this is deregulation of mitochondrial metabolism, with reduced antioxidant response and increased sensitivity to reactive oxidative species (ROS)‐inducing drugs (Rashid et al., 2019).

Tested LSE exerted prominent antimigratory effects on colorectal and cervical cancer cells. In colorectal cancer cells, HCT‐116, treatment induced the strongest prooxidative effect which resulted in the most prominent antimigratory effect.

This cell line is isolated from stage IV of colorectal cancer (Islam et al., 2017); therefore, the prominent effect of LSE on suppression of HCT‐116 motility, as very aggressive type of cancer cells, is significant result of our study.

These results may be due to the presence of phenolic acids, the major component of this extract, that are known to be able to reduce migration and invasion of cancer cells inhibiting the expression of factors related to these mechanisms (Han et al., 2018). The best result of this research indicates that low doses of LSE that suppress the migration of cancer cells do not affect healthy cells, as well as probiotic cultures in plankton nor the formation of biofilm. Subsequent research will be focused on examination of the impact of this treatment on the co‐culture model system. Molecular mechanisms of migration will also be the focus of the following studies.

5. CONCLUSION

Ethanol extract showed to be rich in phenolic compounds. Tested extract showed selective, limited, and strain‐specific antimicrobial and antibiofilm activity regarding tested probiotic strains. Cytotoxic concentrations of this treatment do not affect biofilm formed by probiotic strains. The results of this study indicated that low LSE doses do not affect the investigated probiotic cultures in the planktonic form or their biofilm formation. Besides that, the examined low doses of LSE have a strong antimigratory potential on colorectal and cervical cell lines. The antimigratory effects correlate with the acute oxidative effect of low‐dose treatments detected after 24 h.

AUTHOR CONTRIBUTIONS

Milena M. Jovanović: Conceptualization (lead); investigation (lead); methodology (lead); writing – original draft (lead). Katarina G. Marković: Conceptualization (equal); investigation (lead); methodology (lead); validation (lead). Mirjana Ž Grujović: Conceptualization (equal); investigation (lead); methodology (lead); validation (lead). Jelena Pavić: Investigation (lead); methodology (lead); writing – review and editing (equal). Milan Mitić: Formal analysis (lead); investigation (lead); methodology (lead); writing – review and editing (equal). Jelena Nikolić: Conceptualization (equal); formal analysis (lead); visualization (equal). Dragana Šeklić: Conceptualization (lead); visualization (lead); writing – review and editing (lead).

CONFLICT OF INTEREST STATEMENT

All authors declare no conflict of interest.

ACKNOWLEDGMENTS

We are grateful to Nenad Milosavljević (Mycological society “Šumadija”) for providing and authenticating Laetiporus sulphureus mushroom specimen. This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (CN 451‐03‐68/2022‐14/200124 and 451‐03‐68/2022‐14/200122).

Jovanović, M. M. , Marković, K. G. , Grujović, M. Ž. , Pavić, J. , Mitić, M. , Nikolić, J. , & Šeklić, D. (2023). Anticancer assessment and antibiofilm potential of Laetiporus sulphureus mushroom originated from Serbia. Food Science & Nutrition, 11, 6393–6402. 10.1002/fsn3.3577

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.

REFERENCES

  1. Akinmoladun, A. C. , Obuotor, E. M. , & Farombi, E. O. (2010). Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. Journal of Medicinal Food, 13(2), 441–451. 10.1089/jmf.2008.0292 [DOI] [PubMed] [Google Scholar]
  2. Almeida, I. V. , Cavalcante, F. M. , & Vicentini, V. E. (2016). Different responses of vanillic acid, a phenolic compound, in HTC cells: Cytotoxicity, antiproliferative activity, and protection from DNA‐induced damage. Genetics and Molecular Research, 15(4). 10.4238/gmr15049388 [DOI] [PubMed] [Google Scholar]
  3. Amoah, S. K. , Sandjo, L. P. , Kratz, J. M. , & Biavatti, M. W. (2016). Rosmarinic acid—Pharmaceutical and clinical aspects. Planta Medica, 82(5), 388–406. 10.1055/s-0035-1568274 [DOI] [PubMed] [Google Scholar]
  4. Andrews, J. M. (2005). BSAC standardized disc susceptibility testing method (version 4). Journal of Antimicrobial Chemotherapy, 56(1), 60–76. 10.1093/jac/dki124 [DOI] [PubMed] [Google Scholar]
  5. Bhatt, A. P. , Redinbo, M. R. , & Bultman, S. J. (2017). The role of the microbiome in cancer development and therapy. CA: A Cancer Journal for Clinicians, 67(4), 326–344. 10.3322/caac.21398 [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Blagodatski, A. , Yatsunskaya, M. , Mikhailova, V. , Tiasto, V. , Kagansky, A. , & Katanaev, V. L. (2018). Medicinal mushrooms as an attractive new source of natural compounds for future cancer therapy. Oncotarget, 9(49), 29259–29274. 10.18632/oncotarget.25660 [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chaieb, K. , Kouidhi, B. , Jrah, H. , Mahdouani, K. , & Bakhrouf, A. (2011). Antibacterial activity of thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complementary and Alternative Medicine, 11, 29. 10.1186/1472-6882-11-29 [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cohen, P. A. , Jhingran, A. , Oaknin, A. , & Denny, L. (2019). Cervical cancer. Lancet, 393(10167), 169–182. 10.1016/S0140-6736(18)32470-X [DOI] [PubMed] [Google Scholar]
  9. Damián, M. R. , Cortes‐Perez, N. G. , Quintana, E. T. , Ortiz‐Moreno, A. , Garfias Noguez, C. , Cruceño‐Casarrubias, C. E. , Sánchez Pardo, M. E. , & Bermúdez‐Humarán, L. G. (2022). Functional foods, nutraceuticals and probiotics: A focus on human health. Microorganisms, 10(5), 1065. 10.3390/microorganisms10051065 [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Elekofehinti, O. O. , & Kade, I. J. (2012). Aqueous extract of Solanum anguivi Lam. fruits (African Egg Plant) inhibit Fe2+ and SNP induced lipid peroxidation in Rat's brain—In vitro. Der Pharmacia Lettre, 4(5), 1352–1359. [Google Scholar]
  11. FAO/WHO (World Health Organization) . (2006). Probiotics in food. Health and nutritional properties and guidelines for evaluation. FAO Food and Nutrition Paper, 85, 2. [Google Scholar]
  12. Grujović, M. , Mladenović, K. , Semedo‐Lemsaddek, T. , Laranjo, M. , Stefanović, O. D. , & Kocić‐Tanackov, S. D. (2022). Advantages and disadvantages of non‐starter lactic acid bacteria from traditional fermented foods: Potential use as starters or probiotics. Comprehensive Reviews in Food Science and Food Safety, 21(2), 1537–1567. 10.1111/1541-4337.12897 [DOI] [PubMed] [Google Scholar]
  13. Gryglewski, R. J. , Korbut, R. , Robak, J. , & Świȩs, J. (1987). On the mechanism of antithrombotic action of flavonoids. Biochemical Pharmacology, 36(3), 317–321. 10.1016/0006-2952(87)90288-7 [DOI] [PubMed] [Google Scholar]
  14. Han, Y. H. , Kee, J. Y. , & Hong, S. H. (2018). Rosmarinic acid activates AMPK to inhibit metastasis of colorectal cancer. Frontiers in Pharmacology, 9(68). 10.3389/fphar.2018.00068 [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hassen, S. , Ali, A. A. , Kilaparty, S. P. , Al‐Anbaky, Q. A. , Majeed, W. , Boman, B. M. , Fields, J. Z. , & Ali, N. (2016). Interdependence of DNA mismatch repair proteins MLH1 and MSH2 in apoptosis in human colorectal carcinoma cell lines. Molecular and Cellular Biochemistry, 412(1–2), 297–305. 10.1007/s11010-015-2636-3 [DOI] [PubMed] [Google Scholar]
  16. Hossan, M. S. , Rahman, S. , Bashar, A. , Jahan, R. , Al‐Nahain, A. , & Rahmatullah, M. (2014). Rosmarinic acid: A review of its anticancer action. World Journal of Pharmacy and Pharmaceutical Sciences, 3(9), 57–70. [Google Scholar]
  17. Islam, F. , Haque, M. H. , Yadav, S. , Islam, M. N. , Gopalan, V. , Nguyen, N. T. , Lam, A. K. , & Shiddiky, M. J. A. (2017). An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples. Scientific Reports, 7, 133. 10.1038/s41598-017-00206-8 [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Itharat, A. , Houghton, P. J. , Eno‐Amooquaye, E. , Burke, P. J. , Sampson, J. H. , & Raman, A. (2004). In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. Journal of Ethnopharmacology, 90(1), 33–38. 10.1016/j.jep.2003.09.014 [DOI] [PubMed] [Google Scholar]
  19. Kahieshesfandiari, M. , Nami, Y. , Lornezhad, G. , Kiani, A. , Javanmard, A. , Jaymand, M. , & Haghshenas, B. (2021). Herbal hydrogel‐based encapsulated Enterococcus faecium ABRIINW.N7 improves the resistance of red hybrid tilapia against Streptococcus iniae . Journal of Applied Microbiology, 131(5), 2516–2527. 10.1111/jam.15098 [DOI] [PubMed] [Google Scholar]
  20. Key, T. J. , Bradbury, K. E. , Perez‐Cornago, A. , Sinha, R. , Tsilidis, K. K. , & Tsugane, S. (2020). Diet, nutrition, and cancer risk: What do we know and what is the way forward? BMJ, 368, m511. 10.1136/bmj.m511 [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Khalilov, Q. , Li, L. , Liu, Y. , Liu, W. , Numonov, S. , Aisa, H. A. , & Yuan, T. (2019). Brassinosteroid analogues from the fruiting bodies of Laetiporus sulphureus and their anti‐inflammatory activity. Steroids, 151, 108468. 10.1016/j.steroids.2019.108468 [DOI] [PubMed] [Google Scholar]
  22. Kiani, A. , Nami, Y. , Hedayati, S. , Elieh Ali Komi, D. , Goudarzi, F. , & Haghshenas, B. (2021). Application of Tarkhineh fermented product to produce potato chips with strong probiotic properties, high shelf‐life, and desirable sensory characteristics. Frontiers in Microbiology, 12, 657579. 10.3389/fmicb.2021.657579 [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kiani, A. , Nami, Y. , Hedayati, S. , Jaymand, M. , Samadian, H. , & Haghshenas, B. (2021). Tarkhineh as a new microencapsulation matrix improves the quality and sensory characteristics of probiotic Lactococcus lactis KUMS‐T18 enriched potato chips. Scientific Reports, 11(1), 12599. 10.1038/s41598-021-92095-1 [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Klaus, A. , Kozarski, M. , Niksic, M. , Jakovljevic, D. , Todorovic, N. , Stefanoska, I. , & van Griensven, L. J. L. D. (2013). The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants. International Journal of Food Sciences and Nutrition, 64(5), 599–610. 10.3109/09637486.2012.759190 [DOI] [PubMed] [Google Scholar]
  25. Kosanić, M. , Šeklić, D. S. , Jovanović, M. M. , Petrović, N. , & Marković, S. (2020). Original article ‐ Hygrophorus eburneus, edible mushroom, a promising natural bioactive agent. EXCLI Journal, 19, 442–457. 10.17179/excli2019-2056 [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lee, S. M. , Choi, H. C. , & Hyun, M. K. (2019). An overview of systematic reviews: Complementary therapies for cancer patients. Integrative Cancer Therapies, 18, 1534735419890029. 10.1177/1534735419890029 [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Liou, G. Y. , & Storz, P. (2010). Reactive oxygen species in cancer. Free Radical Research, 44(5), 479–496. 10.3109/10715761003667554 [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Lu, K. , Dong, S. , Wu, X. , Jin, R. , & Chen, H. (2021). Probiotics in cancer. Frontiers in Oncology, 11, 638148. 10.3389/fonc.2021.638148 [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ma, G. , Yang, W. , Zhao, L. , Pei, F. , Fang, D. , & Hu, Q. (2018). A critical review on the health promoting effects of mushrooms nutraceuticals. Food Science and Human Wellness, 7(2), 125–133. 10.1016/j.fshw.2018.05.002 [DOI] [Google Scholar]
  30. Muruzović, M. Ž. , Mladenović, K. G. , Stefanović, O. D. , Vasić, S. M. , & Čomić, L. J. R. (2016). Extracts of Agrimonia eupatoria L. as sources of biologically active compounds and evaluation of their antioxidant, antimicrobial and antibiofilm activities. Journal of Food and Drug Analysis, 24(3), 539–547. 10.1016/j.jfda.2016.02.007 [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Olennikov, D. N. , Tankhaeva, L. M. , & Agafonova, S. V. (2011). Antioxidant components of Laetiporus sulphureus (Bull.: Fr.) Murr. fruit bodies. Prikladnaia Biokhimiia i Mikrobiologiia, 47(4), 462–468. [PubMed] [Google Scholar]
  32. O'Toole, G. , & Kolter, R. (1998). Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: A genetic analysis. Molecular Microbiology, 28(3), 449–461. 10.1046/j.1365-2958.1998.00797.x [DOI] [PubMed] [Google Scholar]
  33. Petrović, J. , Glamočlija, J. , Stojković, D. S. , Ćirić, A. , Nikolić, M. , Bukvički, D. , Guerzoni, M. E. , & Soković, M. D. (2013). Laetiporus sulphureus, edible mushroom from Serbia: Investigation on volatile compounds, in vitro antimicrobial activity and in situ control of Aspergillus flavus in tomato paste. Food and Chemical Toxicology, 59, 297–302. 10.1016/j.fct.2013.06.021 [DOI] [PubMed] [Google Scholar]
  34. Petrović, J. , Papandreou, M. , Glamočlija, J. , Ćirić, A. , Baskakis, C. , Proestos, C. , Lamari, F. , Zoumpoulakis, P. , & Soković, M. (2014). Different extraction methodologies and their influence on the bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill. Food and Function. Royal Society of Chemistry (RSC), 5(11), 2948–2960. 10.1039/c4fo00727a [DOI] [PubMed] [Google Scholar]
  35. Petrović, J. , Stojković, D. , Reis, F. S. , Barros, L. , Glamočlija, J. , Ćirić, A. , Ferreira, I. C. F. R. , & Soković, M. (2014). Study on chemical, bioactive and food preserving properties of Laetiporus sulphureus (Bull.: Fr.) Murr. Food and Function, 5, 1441–1451. 10.1039/c4fo00113c [DOI] [PubMed] [Google Scholar]
  36. Popa, G. , Cornea, C. P. , Luta, G. , Gherghina, E. , Israel‐Roming, F. , Bubueanu, C. , & Toma, R. (2016). Antioxidant and antimicrobial properties of L. sulphureus (Bull.) Murrill. Agriculture and Life Science, 5(1), 168–173. [Google Scholar]
  37. Quigley, E. M. (2013). Gut bacteria in health and disease. Gastroenterology and Hepatology, 9(9), 560–569. [PMC free article] [PubMed] [Google Scholar]
  38. Rashid, S. , Freitas, M. O. , Cucchi, D. , Bridge, G. , Yao, Z. , Gay, L. , Williams, M. , Wang, J. , Suraweera, N. , Silver, A. , McDonald, S. A. C. , Chelala, C. , Szabadkai, G. , & Martin, S. A. (2019). MLH1 deficiency leads to deregulated mitochondrial metabolism. Cell Death & Disease, 10(11), 795. 10.1038/s41419-019-2018-y [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Sánchez, B. , Delgado, S. , Blanco‐Míguez, A. , Lourenço, A. , Gueimonde, M. , & Margolles, A. (2017). Probiotics, gut microbiota, and their influence on host health and disease. Molecular Nutrition & Food Research, 61(1), 1600240. 10.1002/mnfr.201600240 [DOI] [PubMed] [Google Scholar]
  40. Sarker, S. D. , Nahar, L. , & Kumarasamy, Y. (2007). Microtitre plate‐based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42(4), 321–324. 10.1016/j.ymeth.2007.01.006 [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Šeklić, D. , Obradović, A. , Stanković, M. , Živanović, M. , Mitrović, T. , Stamenković, S. , & Marković, S. (2018). Proapoptotic and antimigratory effects of Pseudevernia furfuracea and Platismatia glauca on colon cancer cell lines. Food Technology and Biotechnology, 56(3), 421–430. 10.17113/ftb.56.03.18.5727 [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Šeklić, D. S. , Stanković, M. S. , Milutinović, M. G. , Topuzović, M. D. , Štajn, A. Š. , & Marković, S. D. (2016). Cytotoxic, antimigratory, pro‐and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines. Archives of Biological Sciences, 68(1), 93–105. 10.2298/ABS150427131S [DOI] [Google Scholar]
  43. Sezgin, S. , Dalar, A. , & Uzun, Y. (2019). Determination of antioxidant activities and chemical composition of sequential fractions of five edible mushrooms from Turkey. Journal of Food Science and Technology‐Mysore, 57(5), 1866–1876. 10.1007/s13197-019-04221-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Stepanović, S. , Vuković, D. , Dakić, I. , Savić, B. , & Švabić‐Vlahović, M. (2000). A modified microtiter‐plate test for quantification of staphylococcal biofilm formation. Journal of Microbiological Methods, 40, 175–179. 10.1016/s0167-7012(00)00122-6 [DOI] [PubMed] [Google Scholar]
  45. Xing, F. , Hu, Q. , Qin, Y. , Xu, J. , Zhang, B. , Yu, X. , & Wang, W. (2022). The relationship of redox with hallmarks of cancer: The importance of homeostasis and context. Frontiers in Oncology, 12, 862743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Younis, A. M. , Yosri, M. , & Stewart, J. K. (2019). In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus . Annals of Agricultural Science, 64, 79–87. 10.1016/j.aoas.2019.05.001 [DOI] [Google Scholar]
  47. Zommiti, M. , Feuilloley, M. G. J. , & Connil, N. (2020). Update of probiotics in human world: A nonstop source of benefactions till the end of time. Microorganisms, 8(12), 1907. 10.3390/microorganisms8121907 [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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