Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1987 Mar;83(3):460–463. doi: 10.1104/pp.83.3.460

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii1

James V Moroney 1,2,2, Masahiko Kitayama 1,2, Robert K Togasaki 1,2, N Edward Tolbert 1,2
PMCID: PMC1056384  PMID: 16665269

Abstract

Isolated intact chloroplasts from wall-less mutants of Chlamydomonas reinhardtii accumulate inorganic carbon (Ci) from the medium provided the cells had been adapted to low CO2 photoautotrophic growth conditions. Chloroplasts from cultures grown on high (5%) CO2 or photoheterotrophically with acetate did not accumulate inorganic carbon. Chloroplast Ci accumulation from low CO2 grown cells was light dependent and was inhibited by uncouplers and inhibitors of electron transport. In a model for Ci accumulation by Chlamydomonas, it is proposed that CO2 diffuses into the cell and Ci accumulation occurs in the chloroplast.

Full text

PDF
460

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger M. R., Kaplan A., Berry J. A. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep;66(3):407–413. doi: 10.1104/pp.66.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Belknap W. R. Partial Purification of Intact Chloroplasts from Chlamydomonas reinhardtii. Plant Physiol. 1983 Aug;72(4):1130–1132. doi: 10.1104/pp.72.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Belknap W. R., Togasaki R. K. Chlamydomonas reinhardtii cell preparation with altered permeability toward substrates of organellar reactions. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2310–2314. doi: 10.1073/pnas.78.4.2310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Klein U., Chen C., Gibbs M. Photosynthetic Properties of Chloroplasts from Chlamydomonas reinhardii. Plant Physiol. 1983 Jun;72(2):488–491. doi: 10.1104/pp.72.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miller A. G., Colman B. Evidence for HCO(3) Transport by the Blue-Green Alga (Cyanobacterium) Coccochloris peniocystis. Plant Physiol. 1980 Feb;65(2):397–402. doi: 10.1104/pp.65.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moroney J. V., Husic H. D., Tolbert N. E. Effect of Carbonic Anhydrase Inhibitors on Inorganic Carbon Accumulation by Chlamydomonas reinhardtii. Plant Physiol. 1985 Sep;79(1):177–183. doi: 10.1104/pp.79.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moroney J. V., Tolbert N. E. Inorganic Carbon Uptake by Chlamydomonas reinhardtii. Plant Physiol. 1985 Feb;77(2):253–258. doi: 10.1104/pp.77.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moroney J. V., Wilson B. J., Tolbert N. E. Glycolate Metabolism and Excretion by Chlamydomonas reinhardtii. Plant Physiol. 1986 Nov;82(3):821–826. doi: 10.1104/pp.82.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES