Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1987 Mar;83(3):483–489. doi: 10.1104/pp.83.3.483

Potential-Dependent Anion Transport in Tonoplast Vesicles from Oat Roots 1

Klaus H Kaestner 1, Heven Sze 1
PMCID: PMC1056391  PMID: 16665276

Abstract

Potential-dependent anion movement into tonoplast vesicles from oat roots (Avena sativa L. var Lang) was monitored as dissipation of membrane potentials (Δψ) using the fluorescence probe Oxonol V. The potentials (positive inside) were generated with the H+-pumping pyrophosphatase, which is K+ stimulated and anion insensitive. The relative rate of ΔΨ dissipation by anions was used to estimate the relative permeabilities of the anions. In decreasing order they were: SCN− (100) > NO3− (72) = Cl− (70) > Br− (62) > SO42− (5) = H2PO4− (5) > malate (3) = acetate (3) > iminodiacetate (2). Kinetic studies showed that the rate of Δψ dissipation by Cl− and NO3−, but not by SCN−, was saturable. The Km values for Cl− and NO3− uptake were about 2.3 and 5 millimolar, respectively, suggesting these anions move into the vacuole through proteinaceous porters. In contrast to a H+-coupled Cl− transporter on the same vesicles, the potential-dependent Cl− transport was insensitive to 4,4′-diisothiocyano-2,2′-stilbene disulfonate. These results suggest the existence of at least two different mechanisms for Cl− transport in these vesicles. The potentials generated by the H+-translocating ATPase and H+-pyrophosphatase were nonadditive, giving support to the model that both pumps are on tonoplast vesicles. No evidence for a putative Cl− conductance on the anion-sensitive H+-ATPase was found.

Full text

PDF
483

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentrup F. W., Gogarten-Boekels M., Hoffmann B., Gogarten J. P., Baumann C. ATP-dependent acidification and tonoplast hyperpolarization in isolated vacuoles from green suspension cells of Chenopodium rubrum L. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2431–2433. doi: 10.1073/pnas.83.8.2431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briskin D. P., Thornley W. R., Wyse R. E. Membrane transport in isolated vesicles from sugarbeet taproot : I. Isolation and characterization of energy-dependent, h-transporting vesicles. Plant Physiol. 1985 Aug;78(4):865–870. doi: 10.1104/pp.78.4.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cabantchik Z. I., Knauf P. A., Rothstein A. The anion transport system of the red blood cell. The role of membrane protein evaluated by the use of 'probes'. Biochim Biophys Acta. 1978 Sep 29;515(3):239–302. doi: 10.1016/0304-4157(78)90016-3. [DOI] [PubMed] [Google Scholar]
  4. Churchill K. A., Sze H. Anion-Sensitive, H-Pumping ATPase of Oat Roots : Direct Effects of Cl, NO(3), and a Disulfonic Stilbene. Plant Physiol. 1984 Oct;76(2):490–497. doi: 10.1104/pp.76.2.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Churchill K. A., Sze H. Anion-sensitive, h-pumping ATPase in membrane vesicles from oat roots. Plant Physiol. 1983 Mar;71(3):610–617. doi: 10.1104/pp.71.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gallagher S. R., Leonard R. T. Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots. Plant Physiol. 1982 Nov;70(5):1335–1340. doi: 10.1104/pp.70.5.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jennings M. L. Kinetics and mechanism of anion transport in red blood cells. Annu Rev Physiol. 1985;47:519–533. doi: 10.1146/annurev.ph.47.030185.002511. [DOI] [PubMed] [Google Scholar]
  8. Keifer D. W., Franceschi V. R., Lucas W. J. Plasmalemma Chloride Transport in Chara corallina: Inhibition by 4,4'-Diisothiocyano-2,2'-Disulfonic Acid Stilbene. Plant Physiol. 1982 Nov;70(5):1327–1334. doi: 10.1104/pp.70.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lew R. R., Spanswick R. M. Characterization of Anion Effects on the Nitrate-Sensitive ATP-Dependent Proton Pumping Activity of Soybean (Glycine max L.) Seedling Root Microsomes. Plant Physiol. 1985 Feb;77(2):352–357. doi: 10.1104/pp.77.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lin W. Inhibition of anion transport in corn root protoplasts. Plant Physiol. 1981 Aug;68(2):435–438. doi: 10.1104/pp.68.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MacRobbie E. A. The active transport of ions in plant cells. Q Rev Biophys. 1970 Aug;3(3):251–294. doi: 10.1017/s0033583500004741. [DOI] [PubMed] [Google Scholar]
  13. Martinoia E., Schramm M. J., Kaiser G., Kaiser W. M., Heber U. Transport of anions in isolated barley vacuoles : I. Permeability to anions and evidence for a cl-uptake system. Plant Physiol. 1986 Apr;80(4):895–901. doi: 10.1104/pp.80.4.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perlin D. S., Kasamo K., Brooker R. J., Slayman C. W. Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme. J Biol Chem. 1984 Jun 25;259(12):7884–7892. [PubMed] [Google Scholar]
  15. Pierce W. S., Higinbotham N. Compartments and Fluxes of K, NA, and CL in Avena Coleoptile Cells. Plant Physiol. 1970 Nov;46(5):666–673. doi: 10.1104/pp.46.5.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Randall S. K., Sze H. Properties of the partially purified tonoplast H+-pumping ATPase from oat roots. J Biol Chem. 1986 Jan 25;261(3):1364–1371. [PubMed] [Google Scholar]
  17. Scherman D., Henry J. P. Oxonol-V as a probe of chromaffin granule membrane potentials. Biochim Biophys Acta. 1980 Jun 20;599(1):150–166. doi: 10.1016/0005-2736(80)90064-4. [DOI] [PubMed] [Google Scholar]
  18. Schumaker K. S., Sze H. Decrease of pH Gradients in Tonoplast Vesicles by NO(3) and Cl: Evidence for H-Coupled Anion Transport. Plant Physiol. 1987 Mar;83(3):490–496. doi: 10.1104/pp.83.3.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stone D. K., Xie X. S., Racker E. Inhibition of clathrin-coated vesicle acidification by duramycin. J Biol Chem. 1984 Mar 10;259(5):2701–2703. [PubMed] [Google Scholar]
  20. Sze H., Churchill K. A. Mg/KCl-ATPase of plant plasma membranes is an electrogenic pump. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5578–5582. doi: 10.1073/pnas.78.9.5578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wang Y., Leigh R. A., Kaestner K. H., Sze H. Electrogenic h-pumping pyrophosphatase in tonoplast vesicles of oat roots. Plant Physiol. 1986 Jun;81(2):497–502. doi: 10.1104/pp.81.2.497. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES