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. 1987 Mar;83(3):569–572. doi: 10.1104/pp.83.3.569

H+-ATPase Activity from Storage Tissue of Beta vulgaris1

IV. N,N′-Dicyclohexylcarbodiimide Binding and Inhibition of the Plasma Membrane H+-ATPase

Nancy A Oleski 1,2, Alan B Bennett 1
PMCID: PMC1056406  PMID: 16665290

Abstract

The molecular weight and isoelectric point of the plasma membrane H+-ATPase from red beet storage tissue were determined using N,N′-dicyclohexylcarbodiimide (DCCD) and a H+-ATPase antibody. When plasma membrane vesicles were incubated with 20 micromolar [14C]-DCCD at 0°C, a single 97,000 dalton protein was visualized on a fluorograph of a sodium dodecyl sulfate polyacrylamide gel. A close correlation between [14C]DCCD labeling of the 97,000 dalton protein and the extent of ATPase inhibition over a range of DCCD concentration suggests that this 97,000 dalton protein is a component of the plasma membrane H+-ATPase. An antibody raised against the plasma membrane H+-ATPase of Neurospora crassa cross-reacted with the 97,000 dalton DCCD-binding protein, further supporting the identity of this protein. Immunoblots of two-dimensional gels of red beet plasma membrane vesicles indicated the isoelectric point of the H+-ATPase to be 6.5.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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