Abstract
When Frankia HFPCcI3 was grown in culture at oxygen O2 levels ranging from 2 to 70 kilopascals O2, under nitrogen fixing conditions, nitrogenase activity adapted to ambient pO2 and showed a marked optimum close to growth pO2. Vesicles were thin walled at low pO2 and very thick walled at high pO2. Freeze fracture transmission electron microscopy confirmed that Frankia produces vesicles with outer walls thickened by multiple lipid-like monolayers, in proportion to ambient pO2.
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