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. 1987 Apr;83(4):785–788. doi: 10.1104/pp.83.4.785

Purification and Characterization of Cytosolic NADP Specific Isocitrate Dehydrogenase from Pisum sativum1

Weiting Ni 1, Eugene F Robertson 1, Henry C Reeves 1
PMCID: PMC1056450  PMID: 16665339

Abstract

Cytosolic NADP-specific isocitrate dehydrogenase was isolated from leaves of Pisum sativum. The purified enzyme was obtained by ammonium sulfate fractionation, ion exchange, affinity, and gel filtration chromatography. The purification procedure yields greater than 50% of the total enzyme activity originally present in the crude extract. The enzyme has a native molecular weight of 90 kilodaltons and is resolved into two catalytically active bands by isoelectric focusing. Purified NADP-isocitrate dehydrogenase exhibited Km values of 23 micromolar for dl-isocitrate and 10 micromolar for NADP, and displayed optimum activity at pH 8.5 with both Mg2+ and Mn2+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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